^(147)Pm 诱发免疫细胞凋亡的电镜观察及 CHX 和 Act D 的防护作用  

ELECTRON MICROSCOPIC STUDIES ON 147 Pm INDUCED APOPTOSIS IN IMMUNE CELLS AND THE RADIOPROTECTION OF CHX AND ACT D

作　　者：朱寿彭[1] 张澜生[1] 付强[1] 

机构地区：[1]苏州医学院

出　　处：《辐射防护》1998年第2期134-138,共5页Radiation Protection

基　　金：国家自然科学基金

摘　　要：对裂变产物１４７Ｐｍ照射人淋巴细胞白血病细胞株Ｍｏｌｔ－４细胞和巨噬细胞株Ａｎａ－１细胞诱发的细胞凋亡及其防护因子进行了研究。通过透射电镜的形态观察，发现１４７Ｐｍ可诱发Ｍｏｌｔ－４和Ａｎａ－１两株免疫细胞核断裂，核染质边聚，以及呈现膜包裹着的凋亡小体形成为特征的细胞凋亡。研究表明，蛋白质合成抑制剂ＣＨＸ和ＲＮＡ合成抑制剂ＡｃｔＤ可显著抑制由１４７Ｐｍ诱发的免疫细胞ＤＮＡ链断裂，对１４７Ｐｍ照射诱发的免疫细胞凋亡有明显的防护作用。At present,the apoptosis in Molt 4 cell (a human acute lymphoblastic leukemia cell line) and Ana 1 cell (a macrophage cell line) were studied after internal irradiation with fission product 147 Pm.The morphological changes observed under electron microscope indicated that in Molt 4 and Ana 1 immune cells after incubation with 147 Pm,there were chromatin fragmentation,margination of condensed chromatin and the membrane bounded apototic bodies formation.The findings show that 147 Pm can sigificantly increase the DNA fragmentation in immune cells,which may be inhibited by CHX and Act D.So both CHX and Act D show a protective effect on the 147 Pm induced apoptosis in Molt 4 and Ana 1 cells. (

关 键 词：CHX 防护 辐射损伤 免疫细胞 细胞凋亡 钷147 

分 类 号：R818[医药卫生—放射医学] R142[医药卫生—临床医学]