[C_8mim]Br对蚯蚓抗氧化系统的亚慢性毒性效应  

Subchronic Toxic Effects of 1 Methyl-3-Octylimidazolium Bromide Ionic Liquid on the Antioxidant System of Earthworm

作　　者：罗艳蕊[1] 李效宇[2] 运迷霞[3] 王键吉[4] 

机构地区：[1]河南师范大学体育学院,河南新乡453007 [2]河南师范大学生命科学学院,河南新乡453007 [3]华东师范大学生命科学学院,上海200062 [4]河南师范大学化学与环境科学学院,河南新乡453007

出　　处：《农业环境科学学报》2009年第2期343-347,共5页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(20573034);河南省杰出人才创新工程项目(2006KYCX021)

摘　　要：以OECD标准的基质染毒法测定了离子液体溴化1-辛基-3-甲基咪唑([C8mim]Br)对蚯蚓(Eisenia foetida)的急性毒性效应和亚慢性毒性条件下蚯蚓体内CAT、SOD、GST的活性和GSH、MDA含量的变化,以期初步分析[C8mim]Br对蚯蚓抗氧化系统的作用及其毒性作用的可能机理。结果表明,[C8mim]Br对蚯蚓的7d-LD50和14d-LD50分别为206.8mg.kg-1和159.4mg.kg-1。亚慢性暴露42d后蚯蚓体内CAT的活性受到显著抑制;SOD的活性在低浓度(1～5mg.kg-1)受到抑制,高浓度(20～40mg.kg-1)被激活;在高浓度处理组(20～40mg.kg-1)GST的活性显著高于对照。10～40mg.kg-1浓度的[C8mim]Br处理组GSH的含量显著升高,各处理组MDA含量与对照相比没有差异。推测[C8mim]Br可能通过肠道吸收进入蚯蚓体内,并诱导了蚯蚓体内抗氧化系统的反馈效应。This paper present the results of the acute toxicity determination of 1 methyl-3-oetylimidazolium bromide （[C8mim]Br）ionic liquid on earthworm Eiseniafoetida and responses of the earthworm antioxidant system to the subchronie toxicity of this kind of ionic liquid, in which the activity of CAT, SOD, GST and the content of GSH and MDA were measured to evaluate the possible mechanism of [C8mim]Br to E. foetida. The results showed that：the 7 d and 14 d LDs0 of ionic liquids to E. foetida were 206.8 mg· kg-1 and 159.4 mg· kg-1, respectively. And after 42 days of subehronic exposure to [C8mim]Br, the CAT activity of E. foetida were inhibited significantly, the SOD activity were also in- hibited at low concentrations （ 1-5 mg· kg-1 ）of [C8mim]Br, but＇were stimulated at high concentrations of 20-40 mg· kg-1. As for the activity of GST, it was significantly higher than the control at 20-40 mg· kg-l of [C8mim]Br. The content of GSH increased than the control while the MDA content had no changes after exposure. According to the above results, we assume that [C8mimlBr might be absorbed by the earthworm gut or via the derma of earthworm, and then they disturb the metabolic reaction of earthworm and induce the feedback reaction of earthworm antioxidant system.

关 键 词：溴化1-辛基-3-甲基咪唑离子液体 赤子爱胜蚓 急性毒性 亚慢性毒性 抗氧化系统 

分 类 号：S859.796[农业科学—临床兽医学] X174[农业科学—兽医学]