前列腺素E_1冻干隐形脂质体包封率的测定  

Determination of Entrapment Efficiency of Freeze-dried PGE_1 Stealth Liposomes

作　　者：时念秋[1] 张大同[1] 王东凯[2] 关世侠 陈宇洲[4] 陈修毅 

机构地区：[1]山东轻工业学院化学工程学院,山东济南250353 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]广州瑞济生物技术有限公司,广东广州510730 [4]天津中医药大学中药学院,天津300193 [5]山东医药工业研究所,山东济南250100

出　　处：《食品与药品》2009年第1期42-44,共3页Food and Drug

摘　　要：目的评价前列腺素E1(PGE1)冻干隐形脂质体的质量,测定PGE1冻干隐形脂质体的包封率。方法用超滤法分离脂质体和游离药物;采用Hypersil C18柱(250mm×4.6mm,5μm),流动相:pH4.9的磷酸二氢钾溶液-乙腈(3∶2),流速1.0mL·min-1,检测波长210nm,测定药物含量,计算包封率。结果超滤法能很好的分离脂质体与游离药物,PGE1在1～50mg·L-1内线性关系良好(r=0.9993),平均包封率为93.02%。结论超滤-HPLC法测定PGE1冻干隐形脂质体包封率方法简便、准确。Objective To evaluate the quality of freeze-dried prostaglandin E1（PGE1） stealth liposomes and determine the entrapment efficiency. Methods Liposomes and dissociative drug were separated by ultrafiltration. The content ofPGE1 was determined by Hypersil C18 column （250 mm× 4.6 mm, 5 μm） with the mixture of KH2PO4（PH 4.9）-acetonitrile（3 ： 2） as mobile phase at the flow rate of 1.0 mL · min^-1. The detection wavelength was 210 nm. The entrapment efficiency was calculated. Results It was good to separate liposomes and dissociative drug by ultrafiltration. There was a good linear relationship （r=0.999 3） when the concentration of PGE1 was 1 -50 mg · L^-1. The average entrapment efficiency was 93.02 %. Conclusion The ultrafiltration-HPLC method is convenient and accurate for the determination of entrapment efficiency of freeze-dried PGE1 stealth liposomes.

关 键 词：超滤法 高效液相色谱法 前列腺素E1 隐形脂质体 包封率 

分 类 号：R944.9[医药卫生—药剂学]