哮喘患者血清被动致敏对人气道平滑肌细胞ERK信号传导途径的影响  

Effect of passive sensitization on ERK signal transduction pathway in HASMCs

作　　者：赵丽敏[1] 马利军[1] 张罗献[1] 吴纪珍[1] 

机构地区：[1]河南省人民医院呼吸内科,河南郑州450003

出　　处：《中国现代医学杂志》2009年第3期424-426,共3页China Journal of Modern Medicine

基　　金：河南省科技攻关项目(No:072300450100);河南省卫生厅医学高新技术发展扶持项目(No:20060140)

摘　　要：目的研究哮喘患者血清被动致敏后,细胞外信号调节激酶(extracellular signal regulate dkinase,ERK)磷酸化的变化,以探讨ERK信号传导途径在哮喘发病中的作用。方法免疫细胞化学法及免疫印记法检测ERK蛋白及其磷酸化(p-ERK)水平。结果哮喘患者血清被动致敏后,ERK蛋白的表达无明显变化,而p-ERK的表达明显升高(n=4,P<0.05)。结论哮喘患者血清被动致敏后,HASMCs的ERK磷酸化显著增强,此变化可能是哮喘形成和发生的机制之一。[Objective] To investigate the effect of passive sensitization by asthmatic serum on the extracellular signal regulated kinase （ERK） in human airway smooth muscle cells （HASMCs）. [Methods] Immunoeytochemistry staining and Western-blot techniques were used to detect the protein ERK and level of Phospho-ERK （p-ERK）. [Results] There was no significance in protein ERK between control and passively sensitized groups. But the level of p-ERK in passively sensitized group was significantly higher than：that in control groups （n =4, P 〈0.05）. [Conclusion] P-ERK was significantly increased in HASMCs passively sensitized by asthmatic serum compared with nonsensitized cells. This change might be involved in the mechanisms of the formation and development of asthma.

关 键 词：哮喘 细胞外信号调节激酶 信号传导 

分 类 号：R562.25[医药卫生—呼吸系统]