东方拟无枝酸菌原生质体激光诱变选育  

Breeding of Amycolatopsis orientalis Strain by Protoplast Mutation

作　　者：王成[1] 程冀平[1] 闫敬[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《河北化工》2009年第2期19-20,共2页Hebei Chemical Industry

摘　　要：为了选育去甲基万古霉素高产菌,以东方拟无枝酸菌07-8-32为出发菌株,制作原生质体,经氦氖激光处理后分离。经过21批次的筛选得到的突变株08-8-48效价比出发株高27.3%,且菌丝状态、传代稳定性好。To breed the N-demethylvaneomycin high producing strain, the Amyeolatopsis orientalis 07-8-32 was adopted to make protoplast. The protoplast was mutated by He-Ne laser. Finally, the mutant 08-8-48 was screened through 21 batches, the titer of the mutant 08-8-48 was 27. 3% higher than the titer of the Amycolatopsis orientalis 07-8-32, and the genetic stabilization of the mutant 08-8-48 was fine.

关 键 词：东方拟无枝酸菌 原生质体诱变 氦氖激光 去甲基万古霉素 

分 类 号：R978[医药卫生—药品]