多孔镁表面生物活性β-TCP涂层的制备及其细胞相容性研究  

Preparation and Cytocompatibility of Bioactive β-TCP Coatings on Porous Magnesium Scaffold Surface

作　　者：耿芳[1,2] 谭丽丽[1] 贺永莲[3] 杨敬玉[3] 张炳春[1] 杨柯[1] 

机构地区：[1]中国科学院金属研究所,辽宁沈阳110016 [2]中国科学院研究生院,北京100049 [3]沈阳药科大学,辽宁沈阳110016

出　　处：《稀有金属材料与工程》2009年第2期318-322,共5页Rare Metal Materials and Engineering

基　　金：中国科学院知识创新工程重要方向项目(KGCX2-YW-207)

摘　　要：采用化学沉积法在镁支架表面制备生物活性的β-TCP(磷酸三钙陶瓷)涂层,利用X射线衍射,扫描电子显微镜研究β-TCP涂层的相结构和表面形貌。并对表面改性的镁支架与类成骨细胞UMR106的体外生物相容性进行了详细研究。结果表明,表面改性后的镁支架浸提液细胞毒性为1级,即无细胞毒性;材料浸提液无细胞DNA毒性,对细胞周期无改变,也无异倍体细胞出现。实验结果证明,表面改性后的多孔镁具有良好的细胞相容性,是一种很有前景的新型骨组织工程支架材料。A chemical deposition route was employed to prepare bioactive β-TCP coatings on porous magnesium scaffold surfaces. The phase identification and surface morphology of the specimens were studied by X-ray diffraction and scanning electron microscope, respectively. The cytocompatibility of treated porous magnesium scaffolds was studied in the present paper by using human osteoblast-like UMR106 cells. It was found that the cytotoxicity was in grade 1, indicating cytotoxicity free and without damage to DNA and no change to the cell cycle for the extracted liquid of treated magnesium. All the experimental results indicate that the treated porous magnesium has a good cytocompatibility and potential to be a new type of bone tissue engineering scaffolds.

关 键 词：多孔镁 骨组织工程 Β-TCP 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]