4种自身抗体对间接免疫荧光法检测抗中性粒细胞胞质抗体的影响  

Influence of 4 autoantibodies in detection of antineutrophil cytoplasm antibodies by indirect immunofluorescence technigues

作　　者：罗冰[1] 王艾丽[1] 虞伟[1] 刘国瑞[1] 李梅[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,江苏南京210002

出　　处：《医学研究生学报》2009年第2期124-125,130,I0002,共4页Journal of Medical Postgraduates

基　　金：中国博士后科学基金资助项目(批准号:20060400940);江苏省博士后科学基金资助项目(批准号:0602008C)

摘　　要：目的:观察抗核抗体、抗线粒体抗体、抗高尔基体抗体和抗核糖体P蛋白抗体对间接免疫荧光(ⅡF)法检测抗中性粒细胞胞质抗体(ANCA)结果的影响。方法:用ⅡF法检测4种自身抗体阳性血清可能出现的ANCA荧光图谱;用ELISA法确认上述4种血清的靶抗原。结果:用乙醇固定的中性粒细胞检测ANCA受自身抗体的影响较大,甲醛固定的中性粒细胞受到的影响较小,同时应用猴肝切片可将这4种自身抗体与ANCA区别。结论:联合应用猴肝切片与乙醇和甲醛固定的中性粒细胞抗原片,有助于准确判断ANCA结果。Objective： To compare the infuences of anti-nuclear antibody （ANA）, anti-mitochondrial antibody （AMA）, anti-golgi apparatus antibody and anti-ribosomal P protein antibody on the results of the determination of anti-neutrophil cytoplasm antibodies （ANCA） by indirect immunofluorescence （IIF） assay. Methods： ANA and ANCA were detected by IIF techniques, while the major antigens related to ANCA were determined by ELISA. Results： Autoantibodies exerted more influence on ANCA detection with ethanol-fixed neutrophils than that with formaldehyde-fixed ones. And ANA could be distinguished from ANCA with the substrate of the primate liver. Conclusion： More precise results can be obtained in the detection of ANCA by combined use of the substrate of the primate liver and neutrophils fixed with ethanol and formaldehyde.

关 键 词：抗中性粒细胞胞质抗体 自身抗体 间接免疫荧光法 

分 类 号：R392.11[医药卫生—免疫学]