微量稀释法测定白念珠菌对氟康唑的MIC值及两种药敏试验的比较  

Determination of fluconazole MIC to Cand/da a/b/cans by CLSI M27 - A and comparison of two antifugal susceptibility tests

作　　者：秦晓峰[1,2] 吴建华[1] 黄懿[2] 张丽娟[1] 顾军[1] 

机构地区：[1]第二军医大学附属长海医院皮肤科,上海200433 [2]同济大学附属东方医院皮肤科

出　　处：《中国麻风皮肤病杂志》2009年第2期87-89,共3页China Journal of Leprosy and Skin Diseases

基　　金：973计划课题（批准号2005CB523105）

摘　　要：目的：测定临床分离白念珠菌对氟康唑的耐药。方法：应用1997年美国临床和实验室标准化研究院（CLSI）推荐的微量稀释法（M27-A）对我科真菌实验室分离保存的120株白念珠菌进行了氟康唑的体外药物敏感试验，并对药敏结果进行分析。结果：120株白念珠菌中73株对氟康唑敏感，13株剂量依赖敏感，34株耐药。结论：白念珠菌对氟康唑的敏感性下降，可能与氟康唑的广泛应用有关。Objective： To investigate the MIC of Candida albicans strains isolated clinically to flueonazole. Methods： Antifungal susceptibility testing was performed by the Clinical and Laboratory Standards Institute （CLSI） Reference Broth Microdilution M27 - A. Results： Of 120 strains of Candida albicans, 73 strains of Candida albi-cans were found to be susceptible to fluconazole, 13 strains were susceptible-dose dependent and 34 strains were resistant. Conclusion： Susceptibility of Candida albicans to fluconazole is decreased, which may be related to the extensive applications of fluconazole.

关 键 词：白念珠菌 抗真菌药物敏感试验 最小抑菌浓度 氟康唑 耐药 

分 类 号：R379.4[医药卫生—病原生物学] R978.5[医药卫生—基础医学]