Smad4在肝泡状棘球蚴病小鼠中的表达及其意义  

Expression and significance of Smad4 in mice with hepatic alveolar echinococcosis

作　　者：张亚楼[1] 马海龙[1] 刘辉[1] 齐新伟[1] 刘涛[1] 姜涛[1] 姬风彩[1] 温浩[1] 

机构地区：[1]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054

出　　处：《中华消化外科杂志》2009年第1期47-49,共3页Chinese Journal of Digestive Surgery

基　　金：基金项目：国家自然科学基金（30760228）

摘　　要：目的探讨细胞质信息传递介质Smad4在小鼠肝泡状棘球蚴病灶周围的表达及其意义。方法16只小鼠按随机数字表法分为受试组（人工接种肝泡状棘球蚴）和对照组（注射生理盐水），每组8只。应用免疫组织化学方法检测小鼠肝脏中Smad4蛋白的表达。采用X^2检验对结果进行分析，观察泡状棘球蚴对Smad4蛋白表达的影响。结果Smad4蛋白主要表达于细胞核中，部分表达于细胞质中。小鼠感染泡状棘球蚴后6个月，受试组肝组织Smad4表达明显高于对照组（X^2=19．869，P〈0．05）。受试组肝细胞Smad4蛋白呈阳性表达的数量略多于对照组，且强度比对照组高（X^2=58．310，P〈0．05）。结论病灶周围肝组织和肝细胞中Smad4表达增多，其改变可能在纤维化过程中及免疫逃避中发挥效应。Objective To investigate the expression and significance of Smad4 in peripheral hepatocytes of lesions in mice with hepatic alveolar echinococcosis. Methods Eight mice in the test group were inoculated with alveolar echinococcosis and 8 mice in the control group were injected with normal saline. The expression of Smad4 protein in the hepatic tissue of the mice was detected by immunohistochemistry method, and the data were analyzed by ehi-square test. The effect of alveolar eehinococeosis on the expression of Smad4 protein was investigated. Results Smad4 was detected in cell nuclei and partly in the cytoplasm. Six months after the establishment of the mice model for alveolar echinococcosis, the expression of Smad4 in the hepatic tissue in the test group was significantly higher than in the control group （X^2 = 19. 869, P 〈 0.05 ）. The number of Smad4 with positive expression in the hepatocytes in the test group was slightly higher than in the control group, and the expression intensity of Smad4 in the test group was greater than in the control group （ X^2 = 58. 310, P 〈 0.05 ）. Conclusions The increase of the expression of Smad4 protein in the periphery hepatocytes and tissues of hepatic alveolar echinococcosis lesions may play a role in hepatic cirrhosis and immune evasion.

关 键 词：棘球蚴病 肝 SMAD4蛋白 转化生长因子Β 

分 类 号：R532.31[医药卫生—内科学]