曲古菌素A增强食管癌细胞系EC9706对X射线的辐射敏感性  

Radio-sensitization of esophageal cancer EC9706 cells to X ray by histone deacetylase inhibiter Trichostatin A

作　　者：林晓丽[1] 方华圣[2] 张凤秋[2] 押辉远[3] 秦广雍[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,郑州450052 [2]郑州大学物理工程学院,郑州450052 [3]洛阳师范学院生命科学系,洛阳471022

出　　处：《辐射研究与辐射工艺学报》2009年第1期10-14,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家973课题(2004B3719604)资助

摘　　要：利用流式细胞仪及荧光定量PCR技术,分别检测细胞凋亡率和DNA损伤修复相关基因mRNA表达变化,以探讨曲古菌素A(Trichostatin A,TSA)能否在体外增强食管癌细胞对X射线的敏感性。结果显示,辐射前TSA的处理显著提高了X射线引起的细胞凋亡率(P<0.05);X射线的单独处理显著上调了基因ATM、XRCC2和Lig4(8Gy除外)的mRNA水平(P<0.05),TSA单独和TSA联合X射线都下调了ATM、XRCC2和Lig4的mRNA水平。提示TSA很可能通过下调ATM、Lig4和XRCC2 mRNA水平使食管癌细胞对X射线敏感。Cell apoptosis and the expression of DNA damage repair-related genes XRCC2, ATM and Lig4 were detected separately by flow cytometry analysis and real-time fluorescence quantitative （RT-PCR）, in order to investigate the effect of Trichostatin A（TSA）pre-treatment on the radiosensitivity of esophageal cancer EC9706 ceils to X ray in vitro. The results indicate that pre-irradiation exposure to TSA enhances cell apoptotic rate by X ray markedly （P 〈 0.05） and the mRNA levels of XRCC2, ATM and Lig4（except 8Gy）are up-regulated by X ray individually（P〈0.05）. TSA alone and TSA in combination with X ray all reduce mRNA levels of XRCC2, ATM and Lig4. In conclusions, TSA may radio-sensitize EC9706 cells to X ray by reducing mRNA levels of XRCC2, ATM and Lig4.

关 键 词：曲古菌素A 食管癌细胞 X射线 辐射敏感性 凋亡 

分 类 号：Q279[生物学—细胞生物学] Q691