彗星实验图像分析中彗星分割阈值的选择  

Reasonable threshold value used to segment the individual comet from the comet assay image

作　　者：闫学昆[1] 陈英[1] 杜杰[1] 张学清[1] 骆亿生[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2009年第1期15-19,共5页Journal of Radiation Research and Radiation Processing

基　　金：军队十一五重大专项项目子课题

摘　　要：彗星实验(单细胞凝胶电泳)图像自动分析中,彗星轮廓的合理分割是后续各项指标分析的前提。首先采用几种常用的图像边缘检测算法对彗星轮廓进行提取,并分析了彗星图像的特点;然后对文献中提到的和现有彗星分析软件(如CASP、TriTek CometScoreTM)中的彗星轮廓分割方法进行比较;最后采用一种基于图像内容的阈值分割方法,实现了最佳阈值的选择,并得出了彗星轮廓分割的几点原则。Reasonable segmentation of the individual comet contour from the Comet Assay （CA） images is the precondition for all of parameters analysis during the automatic analyzing for the CA. The Otsu method and several arithmetic operators for image segmentation, such as Sobel, Prewitt, Roberts and Canny were used to segment the comet contour, and characters of the CA images were analyzed firstly. And then the segmentation methods which had been adopted in the software for CA automatic analysis, such as the CASP, the TriTek CometScoreTM, were put for- ward and compared. At last, a two-step procedure for threshold calculation based on image-content analysis is adopted to segment the individual comet from the CA images, and several principles for the segmentation are put forward too.

关 键 词：彗星实验 图像分析 分割阈值 剂量-效应曲线 

分 类 号：Q345.2[生物学—遗传学]