Effect of Acupuncture-moxibustion on TNF-α,sTNFR-Ⅰ and sTNFR-Ⅱ of Rats with Crohn's Disease  

作　　者：施征 马晓芃 吴焕淦 秦秀娣 钱晴兰 张卫 韩丑萍 

机构地区：[1]Shanghai Research Institute of Acupuncture and Meridian

出　　处：《Journal of Acupuncture and Tuina Science》2009年第1期29-32,共4页针灸推拿医学（英文版）

基　　金：National Natural Science Foundation of China (30271638)

摘　　要：Objective： To observe the effect of acupuncture-moxibustion on TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in rats with Crohn＇s disease （CD）. Method： The models of CD rats induced by TNBS were randomized into model group, herbal cake-partitioned moxibustion group and electroacupuncture group as well as a normal control group. The histopathological changes of colon mucus membrane were observed with HE staining and the contents of TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ were detected with ELISA method. Results： When compared with the normal group, the TNF-α level in CD rats was substantially elevated and the sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. After herbal cake-partitioned moxibustion and electroacupuncture treatment, the TNF-α level in CD rats was substantially reduced and sTNFR-Ⅰ and sTNFR-Ⅱ showed no marked changes. The inflammatory lesions and abnormal structures of CD rats were significantly improved after herbal cake-partitioned moxibustion and electric stimulation. Conclusion： Herbal cake-partitioned moxibustion and electroacupuncture can both remarkably reduce the TNF-α level in blood serum, and this might be one of the action mechanisms in the treatment of CD.目的:观察针灸对克罗恩病大鼠血清TNF-α、sTNFR-Ⅰ、sTNFR-Ⅱ的影响。方法:采用TNBS制备大鼠克罗恩病模型,随机分为模型组、隔药灸组、电针组,并与正常大鼠作对照,采用HE染色观察结肠黏膜组织病理学变化,应用ELISA方法检测血清TNF-α、sTNFR-Ⅰ、sTNFR-Ⅱ的含量。结果:与正常组比较,CD大鼠血清TNF-α显著升高、sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。经隔药灸和电针治疗后血清TNF-α水平显著降低,sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、电针治疗后得到明显改善。结论:隔药灸与电针均能够显著降低血清TNF-α的含量,该作用可能是隔药灸与电针治疗CD的作用机制之一。

关 键 词：Crolm＇s Disease Acupuncmre-moxibustion Therapy Tumor Necrosis Factor-alpha Receptors  Tumor Necrosis Factor  Type Ⅰ Receptors  Tumor Necrosis Factor  Type Ⅱ RATS 

分 类 号：R2-03[医药卫生—中医学]