远红外线对造血干/祖细胞生物学特性的影响  

Effect of Far Infrared Ray on the Characteristic of the Hematopoietic Cell

作　　者：陈婧婧[1] 李兴玉[1] 李文信[2] 吴胜伟[2] 李静[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国科学院应用物理研究所,上海201800

出　　处：《现代生物医学进展》2009年第3期455-459,共5页Progress in Modern Biomedicine

基　　金：中国科学院核分析重点实验室资助

摘　　要：目的:了解远红外线对造血细胞增殖和定向分化的影响方法:应用体外小鼠骨髓细胞培养技术,观察远红外线对粒-单祖细胞(CFU-GM)、红系祖细胞(CFU-E)和成纤维细胞(CFU-F)增殖和分化的影响,并且利用免疫荧光纳米粒子(IFNB)检测靶细胞表面分化抗原(CD),进一步评估远红外线对造血干/祖细胞的影响结果:远红外线在37℃条件下照射对CFU-E、CFU-F和CFU-GM生成有促进作用,小鼠骨髓细胞表面标记有变化,靶细胞经2min照射后,增殖最旺盛2 min组与其它实验组(1 min,5 min,10min)数据经统计学处理,具有差异性(P均<0.05)。Objective： To explore the effect of far infrared （FIR） on hematopoietic cell proliferation and differentiation. Methods： Mouse bone marrow cell culture in vitro was used to observe the effects of far infrared on the proliferation and differentiation of colony-forming units granulocyte-macrophage （CFU-GM）, colony forming unit-erythroid （CFU-E） and marrow fibroblast colony （CFU-F）. Target cell surface differentiation antigen （CD） was detected by immunofluorescence nanoparticles （IFNB）. Results： The for- mation of CFU-E, CFU-F and CFU-GM have been promoted, and mouse bone marrow cell-surface markers have changed at 37℃ with the far infrared radiation. The proliferation of target cells was the most exuberant after 2 min radiation with FIR. There was significant difference in statistical data between the 2 min group and the experimental groups （1 min, 5min, and 10min）. Conclusions： Far infrared has positive control on the proliferation of mouse bone marrow hematopoietic stem/progenitor cells and can induce cell surface markers to change.

关 键 词：远红外线 造血干/祖细胞 正调节 CD表达 

分 类 号：Q345[生物学—遗传学] Q691