小麦种子蛋白图谱鉴定技术的应用  

APPLICATION OF SEED PROTEIN ELECTROPHOREGRAM TECHNIQUE TO WHEAT CULTIVAR IDENTIFICATION

作　　者：孙致良[1,2] 梁永平[1,2] 董少君 谭翠英 王晗[1,2] 

机构地区：[1]莱阳农学院农学系 [2]枣庄市中区农业局

出　　处：《莱阳农学院学报》1998年第1期10-12,共3页Journal of Laiyang Agricultural College

摘　　要：采用一种新型电泳方法，研究了山东省小麦生产上重要品种的电泳图谱特征。莱州９５３、鲁麦１４、鲁麦１５、烟农１５和鲁麦１号品种蛋白图谱的特征谱带分别是１．４５ｃｍ、１．６５ｃｍ、６．１５ｃｍ、７．１５ｃｍ和５．５５ｃｍ。它们的图谱符合系数在４９．３５％～６８．７５％。这种电泳技术分离性能强，能将来自同一组合的鲁麦５号、鲁麦８号和鲁麦１１相互区别开。这３个品种的符合系数高达６０％～７１．８８％，表明遗传上非常相似。这些在小麦种子检验和杂交育种中将有着重要意义。The electrophoregrans of currently important cultivars in Shandong were characterized by a new protein electrophoresis method. It was revealed that the unique bands were 1.45cm,1.65cm,6.15cm,7.15cm and 5.55cm for Laizhou 953,Lumai 14,Lumai 15,Yannong 15 and Lumai 1,respectively. The coefficients of pattern homology between them ranged from 49.35% to 68.75%.The superb separation power of the method could distinguish between Lumai 5,Lumai 8 and Lumai 11 bred from a same cross.All three cultivars had a high average pattern homology of about 68%,suggesting a close genetic relationship. The findings would be Significant in wheat seed testing and breeding.

关 键 词：小麦 电泳图谱 特征谱带 种子 检验 

分 类 号：S512.103.2[农业科学—作物学]