加味三拗汤平喘作用及对肺功能影响的实验研究  

Experimental study on the antiasthmatic effect and impact on pulmonary function of Jiaweisan'ao Decoction

作　　者：赵琰[1] 屈会化[1] 张晗睿[1] 崔健[1] 谢婷[1] 李宇航[1] 王庆国[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《北京中医药大学学报》2009年第1期21-24,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家863计划资助项目(No.2004AA2Z3012);教育部新世纪优秀人才培养计划项目(No.NCET-04-0140)

摘　　要：目的观察加味三拗汤平喘作用及对肺功能的影响,为临床使用加味三拗汤治疗哮喘提供实验依据。方法分别制备卵蛋白致敏豚鼠模型和卵蛋白致敏大鼠模型各70只,随机分为5组,即哮喘模型组、加味三拗汤小、中、大剂量组(分别是成人用量的4、8、16倍)及地塞米松阳性对照组[1mg/(kg·d)],每组各14只;另设正常组给予注射生理盐水作为对照。记录致敏豚鼠每次激发的引喘潜伏期,测定致敏大鼠的肺功能指标。结果加味三拗汤和阳性药地塞米松均能延长卵蛋白致敏豚鼠哮喘的引喘潜伏期。与模型组相比,加味三拗汤中剂量组有极显著差异(P<0.01),小剂量组和地塞米松阳性对照组有显著差异(P<0.05),大剂量组引喘潜伏期延长,但与模型组相比没有显著差异(P>0.05)。肺功能检测数据表明,各组之间呼气阻力(Re)、吸气阻力(Ri)、肺顺应性(C1)、最大通气量(MVV)、用力呼气中期流量(PEF25%～75%)、用力呼气峰流量(PEF)无明显差异(P>0.05)。用力肺活量(FVC)模型组显著低于正常组(P<0.01),各给药组明显高于模型组(加味三拗汤小、中、大剂量组P<0.05,阳性对照组P<0.01);第0.2秒用力呼气容量占用力肺活量百分率(FEV0.2/FVC),模型组显著低于正常组(P<0.01),加味三拗汤小、中、大剂量组和阳性对照组显著高于模型组(P<0.01)。结论加味三拗汤在解除致敏豚鼠支气管平滑肌痉挛、减轻哮喘症状方面有一定的优势,并能改善致敏哮喘大鼠的肺通气功能。Objective To observe the antiasthmatic effect and impact of Jiaweisan＇ ao Decoction on pulmonary function, and to investigate the experimental perspective of antiasthmatic effect of Jiaweisan＇ ao Decoction （JWSAD）. Methods Seventy Sprague-Dawley （SD） rats and Seventy guinea pigs were randomly divided into five groups： model control group, （n =14）; low dose group, （n =14）; medium dose group, （n =14）; high dose group, （n = 14）, respectively corresponding 4, 8 and 16 times with the adult dosage; dexamethasone （DEX） group, （n = 14, 1 mg/kg/d）. The asthmatic model were sensitized by intraperitoneal injection of OVA and challenged with nebulized OVA, and control group were only intraperinoeal injected with normal saline. Every asthmatic latency periods and pulmonary function parameters were recorded. Result Both JWSAD and DEX prolonged the specific asthmatic latent period in OVA-challenged guinea pigs. Compared to model control group, low and medium dose group showed significant difference （respective, P 〈 0.01, P 〈 0. 05）, while there was no significant difference between high dose and control group （P 〉0.05）. Pulmonary parameters indicated that there was no significant difference among inspiration resistance （Ri）, expiration resistance（Re）, lung compliance（C1）, maximal voluntary ventilation（MVV）, forced expiration flow 25% - 75% （FEF 25% -75% ）, peak expiratory flow （PEF）, （P 〉0.05）. While, forced vital capacity （FVC） of model control model was significantly decreased when compared with normal group （P 〈 0. 01 ）, which of administration groups （low, medium, high dose of JWSAD and DEX groups） were significantly increased when compared with model control group（P 〈0.05 for all JWSAD groups, P 〈0.01 for DEX group）; compared to normal group, forced expiratory volume in 0.2s/forced vital capacity （FEV0.2/FVC） of model control group was significantly decreased （ P 〈 0. 01 ）, and FEV0. 2/FVC of administration gro

关 键 词：加味三拗汤 平喘 肺功能 豚鼠 大鼠 

分 类 号：R285.5[医药卫生—中药学]