健脾补肾解毒方对小鼠Lewis肺癌的抑制及A549细胞周期的影响  

Effect of Jianpibushenjiedu Formula on mice Lewis lung carcinoma and cell cycle of A549 lung carcinoma cells

作　　者：曹勇[1] 孟金成[1] 夏清华[1] 孟华[1] 陈璐[1] 许林利[1] 李亮[1] 

机构地区：[1]暨南大学医学院中医系,广东510632

出　　处：《北京中医药大学学报》2009年第1期38-41,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：广东省科技计划项目(No.73062);广州市科技计划项目(No.2007Z3-E5091);广东省中医药管理局项目(No.2040030)

摘　　要：目的探讨健脾补肾解毒方对肺癌的抑制作用及机理。方法以Lewis肺癌移植小鼠建立小鼠Lewis肺癌模型,随机分为:模型对照组,健脾补肾解毒方大、中、小剂量组,顺铂组,大、中、小剂量分别加顺铂组。并以健脾补肾解毒方大、小剂量组和模型对照组制备荷瘤动物药物血清,采用流式细胞术,分别观察健脾补肾解毒方及其药物血清对小鼠Lewis肺癌的抑制和对人肺腺癌A549细胞周期的影响。结果健脾补肾解毒方对小鼠Lewis肺癌有一定的抑制作用,能增强顺铂对肺癌细胞的抑制杀伤作用,健脾补肾解毒方药物血清能显著阻滞细胞于G0/G1期,减少S期细胞的分布(P<0.01)。结论健脾补肾解毒方具有抑瘤增效和抑制细胞增殖作用,其机理可能与其抑制肺癌细胞增殖周期有关。Objective To study the effect of Jianpibushenjiedu Formula （JPBSJDF） on lung carcinoma and its mechanism. Methods The mice-transplanted Lewis lung cancer were randomly divided into model control group; JPBSJDF high dosage group, medium dosage group and low dosage group;eisplatin（DDP） group;JPBSJDF high dosage , medium and low dosage combined respectively with DDP group. The drugserum with JPBSJDF high and low dosage group, and model control group from tumor-beating animals were prepared. The effect of JPBSJDF on antitumor of mice Lewis lung carcinoma and its drug-serum on cell cycle of human lung carcinoma A549 cells were observed respectively. Results JPBSJDF had certain inhibitory effect on mice Lewis lung carcinoma, and enhanced inhibitary and lethal effect of DDP to lung carcinoma cells. JPBSJDF drug-serum could significantly block the A549 cells at the Go -G1 phase, which decreased cell ratio in the S phase （P 〈 0.01 ）. Conclusion JPBSJDF has effect of antitumor and synergy. Tumor cell cycle inhibiting probably contributes to this effect.

关 键 词：健脾补肾解毒方 LEWIS肺癌 A549细胞周期 小鼠 

分 类 号：R285.5[医药卫生—中药学]