降糖明目汤对糖尿病小鼠视网膜促血管生成因子的影响  

Influence of Jiangtangmingmu Decoction on vascular growth factors in the retinas of diabetic mouse

作　　者：罗丽华[1] 侯艳丽[1] 卢清君[2] 王艳玲[1] 

机构地区：[1]首都医科大学附属北京友谊医院眼科,北京100050 [2]北京市眼科研究所

出　　处：《北京中医药大学学报》2009年第1期47-49,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：首都医科大学基础-临床合作基金项目(No.2006jl22)

摘　　要：目的探讨降糖明目汤对C57BL/6小鼠糖尿病模型视网膜中血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)表达的影响。方法将实验动物随机分为正常对照组、糖尿病模型组、降糖明目汤治疗组,采用链脲佐菌素诱导C57BL/6小鼠糖尿病模型,给药3月后取各组小鼠新鲜视网膜组织进行Western-blot蛋白定量,比较各组间VEGF、bFGF表达差异。结果降糖明目汤治疗组小鼠视网膜VEGF和bFGF含量较糖尿病模型组明显减少(P<0.05),但较正常对照组增加(P<0.05)。结论降糖明目汤对糖尿病视网膜病变的治疗作用可能与其能降低糖尿病小鼠视网膜VEGF和bFGF的含量,抑制其表达有关。Objective To investigate the influence of Jiangtangmingmu Decoction on the expression of vascular endothelial growth factor （VEGF） and basic fibroblast growth factor （bFGF） in the retinas of diabetic mouse. Methods The mice were randomly divided into normal control group（ group A） ,diabetes model group （group B） and Jiangtangmingmu Decoction treatment group （group C）. The mouse model of diabetic was established by abdominal injection of streptozotocin. After 3 months, all mice were killed and the expression of VEGF and bFGF in the retina were detected by western blot. Results The expressions of VEGF and bFGF in the Jiangtangmingmu Decoction treatment group were lower than that in the diabetic model group, but higher than that in the normal control group, both with significant differenees（P 〈0.05, P 〈 0.05）. Conclusions Jiangtangmingmu Decoction has the therapeutic effect on diabetic retinopathy, which may be related to inhibiting the expression of VEGF and bFGF in retinas of diabetic mouse, the expression of VEGF and bFGF in retinas of diabetic mouse.

关 键 词：血管内皮生长因子 碱性成纤维细胞生长因子 糖尿病视网膜病变 降糖明目汤 小鼠 

分 类 号：R285.5[医药卫生—中药学]