Pax-8基因在心脏发育中蛋白表达下调  

Down-regulated protein expression level of Pax-8 gene during heart development in mice

作　　者：倪秋明[1] 章佳颖[1] 来丹丹[1] 褚茂平[1] 王赛斌[1] 黄晓燕[1] 张怀勤[1] 杨德业[1] 

机构地区：[1]温州医学院附属第一医院心血管内科温州医学院心血管生物和基因研究所,浙江温州325000

出　　处：《温州医学院学报》2009年第1期1-4,共4页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30571050);温州市科技发展计划基金资助项目(Y2006A017)。

摘　　要：目的:观察Pax-8基因在ALK3基因敲除小鼠心脏中的蛋白表达情况,探讨Pax-8基因在心脏发育中的作用。方法:基因芯片(microarray)和定量逆转录-聚合酶链反应测定ALK3基因的下游基因,免疫组化法检测13.5 d小鼠胚胎心脏特异性ALK3基因敲除小鼠胚胎中Pax-8蛋白的表达,连续病理切片及HE染色观察刚出生的Pax-8基因敲除小鼠的心脏形态。结果:在心脏特异的ALK3基因敲除小鼠中,转录因子Pax-8基因mRNA的表达水平上被下调7.1倍。免疫组化结果显示:13.5 d小鼠胚胎心脏特异性ALK3基因敲除的纯合子小鼠Pax-8基因在蛋白水平与杂合子相比也明显下调。Pax-8基因敲除小鼠纯合子有明显的室间隔和左心室肥厚,心脏呈球形。结论:Pax-8基因是ALK3基因的下游基因,与心脏发育有密切关系。Objective： To investigate the level of Pax-8 protein expression in the conditional ALK3 kockout mice heart and study the role of Pax-8 gene in the cardiac development. Methods： The microarray and real time quantitative RT-PCR were used to investigate the downstream genes of ALK3. Immunohistochemical technology was used to detect the level of Pax-8 protein expression in the 13.5 d heart derived from a-MHC Cre＋/-,ALK3 F/- and a-MHC Cre＋/-, ALK3 F/＋ mice. To study the contribution of Pax-8 gene in the cardiac development,the heart derived from pax-8 kockout mice was overviewed using consecutive pathological section and HE staining method. Results：The level of Pax-8 mRNA expression was down-regulated 7.1 fold in the embryonic heart （11.5 days） of a-MHC Cre＋/-, ALK3 F/-. The level of Pax-8 protein expression was also remarkably down-regulated in the embryonic heart （13.5 days） of a-MHC Cre＋/-,ALK3 F/-. The heart of pax-8 KO-/-mice became spheroidal,the left ventricle enlarged, left ventricular wall and interventricular septum thickened, papillary muscles in the left ventricle enlarged. Conclusion： Pax-8 gene is the cardiac-specific ALK3 downstream gene. Pax-8 may play important roles in the cardiac development.

关 键 词：Pax-8基因 室间隔缺损 免疫组化 蛋白表达 

分 类 号：R542.2[医药卫生—心血管疾病]