高纯度分离睾丸支持细胞的简便方法  

A Simple Method for Isolating Highly Purified Mouse Sertoli Cells

作　　者：唐丽娟[1] 王庆忠[2] 徐承水[1] 

机构地区：[1]曲阜师范大学生命科学院,曲阜273165 [2]潍坊学院生命工程学院,潍坊261061

出　　处：《细胞生物学杂志》2009年第1期123-124,共2页Chinese Journal of Cell Biology

基　　金：山东省自然科学基金(No.Y2007079);潍坊市科技发展计划项目(No.2007028)资助~~

摘　　要：为寻求简便经济的方法分离培养小鼠睾丸支持细胞,用改良的两种消化液依次消化2～7天的乳鼠睾丸获得细胞悬液,采用反复离心和反复贴壁的方法除去生精细胞,从而获得高纯度的小鼠睾丸支持细胞。To isolate and culture the Sertoli cells from 2-7 days pup mouse, the testis cell suspension was prepared by using two types of digestion solutions called Digestion I and Digestion II. Repeated centrifugation and repeated adherence selection were used to remove germ cells. Finally, highly purified mouse Sertoli cells were obtained.

关 键 词：小鼠 支持细胞 分离 培养 

分 类 号：Q813.11[生物学—生物工程]