卡那霉素与苯胺蓝反应的电化学研究及其应用  

Electrochemical Study on the Reaction of Kanamycin with Aniline Blue and Its Application

作　　者：王学亮[1] 赵杰[1] 

机构地区：[1]菏泽学院化学与化工系,菏泽274015

出　　处：《理化检验（化学分册）》2009年第2期207-209,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：在pH3．0的B-R缓冲溶液中，苯胺蓝在静汞电极上于-0．355V产生明显的还原峰。当加入卡那霉素之后，卡那霉素与苯胺蓝发生作用生成新的离子复合物，使苯胺蓝在-0．355V处的还原峰电流下降，研究发现其降低值△Ip与卡那霉素的质量浓度在5．0～65．0mg·L^-1范围内呈线性关系，线性回归方程为△Ip=-1．028＋0．567C，相关系数为0．998，检出限（3S／N）为1．0mg·L^-1。用该法测定了市售硫酸卡那霉素的含量，所得结果与分光光度法的测定结果一致。In a B-R buffer solution of pH 3. 0, a significant reduction peak of aniline blue with its potential at -0. 355 V was observed at the static mercury electrode. Upon the addition of an appropriate amount of kanamycin to this reaction system, the peak current （Ip） at -0. 355 V was decreased due to the reaction of aniline blue with kanamycin forming an ion-association complex. The magnitude of decrease in peak current （Alp） at -0. 355 V was found to keep linear relationship with the concentration of kanamycin in the range from 5. 0 to 65. 0 mg · L^-1 , corresponding to the linear regression equation of △lp = -1. 028＋0. 567 C with its correlation coefficient of 0. 998. Detection limit （3S/N） found was 1.0 mg ·L^-1. The proposed method was used to determine content of kanamycin in samples of kanamycin injections, giving results in consistency with the results obtained by spectrophotometry.

关 键 词：伏安法 苯胺蓝 卡那霉素 

分 类 号：O657.1[理学—分析化学]