CYP2E1和CYP1A1及白细胞介素-4基因多态性与三氯乙烯药疹样皮炎的关系  

Relationship between gene polymorphism of CYP2E1, CYP1A1, IL-4 and medicamentosa-like dermatitis induced by trichloroethylene

作　　者：徐新云[1] 陈冠豪[1] 武南[1] 庾蕾[1] 黄芳[1] 杨淋清[1] 

机构地区：[1]深圳市疾病预防控制中心毒理室,518020

出　　处：《中华劳动卫生职业病杂志》2009年第2期71-73,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：深圳市科技计划资助课题（JH200505300515A）

摘　　要：目的研究代谢酶基因CYP2E1和CYP1A1以及白细胞介素（IL）-4的基因多态性与三氯乙烯（TCE）药疹样皮炎易感性的关系。方法选择35例TCE药疹样皮炎病例作为病例组，选无皮肤损害的35名健康工人作为对照组。应用实时荧光定量聚合酶链反应（PCR）和TaqMan MGB探针技术，检测病例组和对照组CYP2E1、CYP1A1和IL-4基因的单核苷酸多态性（SNP），计算病例组和对照组的基因型与等位基因型频率。结果CYP1A1基因（rs1048943）的SNP多态性检测结果显示，病例组G等位基因频率（37．1％）明显高于对照组，差异有统计学意义（P〈0．01）；检测发现，病例组CYP2E1基因-1053C→T位点T等位基因频率（41．4％）明显高于对照组，差异有统计学意义（P〈0．01）；对IL-4基因-588C—T位点（rs2243250）检测发现，病例组TT纯合突变频率（75．0％）明显高于对照组，差异有统计学意义（P〈0．01），T等位基因频率（87．5％）明显高于对照组，差异有统计学意义（P〈0．01）。结论CYP1A1、CYP2E1和II-4基因的某些位点的改变可能与少数TCE敏感个体对接触TCE引起的超敏反应存在密切关系，CYP1A1、CYP2E1和IL4的基因多态性可能是TCE药疹样皮炎患者易感性差异相关的遗传学因素之一。Objective To investigate the relationship between gene polymorphism of CYP2E1, CYP1A1, IL-4 and susceptibility of medicamentosa-like dermatitis induced by trichloroethylene （TCE）. Methods 35 patients with medicamentosa-like dermatitis induced by TCE were chosen as the patient group, and 35 healthy workers as control group. The real-time quantitative polymerase chain reaction （PCR） with TaqMan minor groove binding （MGB） probes was used to test single nucleotide polymorphisms （SNP） of CYP2E1, CYP1A1 and IL-4 in the patients with medicamentosa-like dermatitis as well as in the control. The genotypes and the frequency of genotype or allele were compared between the patients and control with statistical analysis. Results The frequency of allele G within CYP1A1 gene （rs1048943） was significantly higher in TCE patients （37.1%） than that in control （P〈0.05） ;the frequency of allele T within CYP2E1- 1053 C/T was significantly higher in TCE patients （41.4%） than that in control （P〈0.01）;the frequency of T/T within IL-4-588 C/T （rs2243250）was significantly higher in TCE patients （75.0%） than that in control （P〈0.01）, and the frequency of allele T within IL-4-588 C/T （rs2243250）was also significantly higher in TCE patients （87.5%） than that in control （P〈0.01）. Conclusion The gene polymorphism of CYP2E1, CYP1A1, IL-4 is probably associated with hypersensitivity for the TCE patients with medicamentosa-like dermatitis, and could be one of the genetic factors related to the individual susceptibility to TCE exposure.

关 键 词：三氯乙烯 药疹样皮炎 细胞色素P450 细胞因子 基因多态性 免疫毒性 

分 类 号：R686[医药卫生—骨科学]