GnRH-A免疫对兔去势效果的实验研究  

Experimental Research on Efficacies of GnRH-A Immunocastration in Rabbits

作　　者：魏锁成[1] 巩转娣[2] 

机构地区：[1]西北民族大学动物医学研究所,兰州730030 [2]西北民族大学医学院附属医院,兰州730030

出　　处：《畜牧兽医学报》2009年第2期191-196,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家民委2002年度重点资助项目(MW2002-ZD-010)

摘　　要：探讨促性腺激素释放激素类似物（GnRH—A）对动物免疫去势的效果和作用机理。用自制的GnRH—A抗原乳剂在30只兔（EG-I与EG-Ⅱ组）的颈背部皮下分2～3点注射1．0mL（100／lg·mL^-1）GnRH—A抗原，EG-Ⅱ组3周后加强免疫1次。测定睾丸长度和质量，体质量变化，GnRH抗体效价与血清睾酮浓度。制备的抗原无菌性、安全性和物理性状良好，EG-Ⅱ组睾丸长度与EG-I组差异极显著（P〈o．01）；EG-Ⅱ组的抗体水平也明显高于EG-I组；免疫注射后2个试验组与对照组的血清睾酮浓度差异逐渐增加，102d时EG-Ⅱ组血清睾酮浓度极显著低于对照组（P〈0．01），且第28天后EG-Ⅱ组显著低于EG-I组（P〈O．05）；EC-Ⅱ组雄兔的体质量和平均日增质量均最大，显著高于EG-I组和对照组（P〈O．05）。GnRH—A免疫家兔对睾丸发育、血清GnRH抗体效价和睾酮浓度具有显著的影响，加强免疫效果更理想。To investigate the efficacies and mechanisms of immunocastration by using gonadotro- pin releasing hormone analogues （GnRH-A） in animals. The GnRH-A antigen emulsion was made by adding incomplete Freund＇s adjuvant after GnRH-A was combined with bovine serum albumin（BSA）. 30 rabbits were injected with GnRH-A antigen, the testis length and weight as well as body weight of the immunized rabbits were measured, GnRH antibody titer and testoster one concentration were detected through ELISA. There was a significant difference of the testis lengths between two EGs groups（P〈0.01） ,antibody levels of EG-Ⅱ group was higher obviously than that of EG- I ; the serum testosterone concentration between EG- I and EG- Ⅱ groups were remarkably different from 7 to 16 weeks（P〈0.05 ）, the body weight and average daily gain of EG- Ⅱ group were the biggest and significantly higher than that of EG- I group as well CG（P〈0.05）. The testis development,serum GnRH antibody titer and testosterone concentration of experiment rabbits changed obviously after immunization of GnRH-A and adding another injection would strengthen castration effects.

关 键 词：促性腺激素释放激素类似物(GnRH—A) 抗原 睾酮 免疫去势 兔 

分 类 号：S829.1[农业科学—畜牧学]