猪繁殖与呼吸综合征病毒和猪圆环病毒2型共感染对猪体内病毒动态及免疫反应的影响  

Effects of Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2 Co-infection on Virological Kinetics and Immunological Responses in Piglets

作　　者：施开创[1] 杨汉春[1] 郭鑫[1] 何宛仑[1] 盖新娜[1] 陈艳红[1] 查振林[1] 

机构地区：[1]中国农业大学动物医学院农业部预防兽医学重点开放实验室,北京100193

出　　处：《畜牧兽医学报》2009年第2期228-234,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展规划(973计划)项目(2005CB523204)

摘　　要：将猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)单感染和共感染6周龄健康仔猪,采用实时定量PCR(Real-ti me PCR)对血清、组织(心、肝、肺、肾、胰、脾、胸腺、扁桃体、腹股沟淋巴结、肠系膜淋巴结)中的PRRSV和PCV2载量进行检测,分别采用间接ELISA和间接IFA对血清中的PRRSV和PCV2特异性抗体进行检测,采用MTT法对猪外周血淋巴细胞(PBLC)的增殖能力进行检测。结果,PRRSV/PCV2共感染组血清、组织中PRRSV的载量均显著高于PRRSV单感染组,PCV2的载量均显著高于PCV2单感染组;共感染组PRRSV和PCV2特异性抗体阳转的时间均晚于并且效价均显著低于PRRSV或PCV2单感染组;共感染组和单感染组PBLC的增殖能力均受到抑制,其严重程度依次为:PRRSV/PCV2组>PRRSV组>PCV2组。由此表明,PRRSV和PCV2在猪体内对彼此的增殖具有促进作用;PRRSV和PCV2共感染对猪的免疫抑制具有加重作用。To explore the interaction between porcine reproductive and respiratory syndrome virus （PRRSV） and porcine circovirus type 2 （PCV2） in pathogenesis, the virological kinetics and immunological responses in piglets to PRRSV and PCV2 co-infection were analyzed. Twenty health- y, six-week-old piglets were randomly divided into control group, PRRSV or PCV2 infected group and PRRSV/PCV2 co infected group with 5 piglets each. After infection, serum and different tissue samples, including heart, liver, lung, kidney, pancreas, spleen, thymus, tonsil, inguinal lymph node and mesenteric lymph node, were used for PRRSV and PCV2 quantitation by real-time PCR, and serum was used for PRRSV-and PCV2-speeific antibody deteetion by indirect ELISA and indirect IFA, respectively. The proliferation activities of peripheral blood lymphocytes （PBLC） were detected by 3-[-4, 5-dimethylthiazol]-2, 5-diphenyltetrazolium bromide （MTT）. The results showed that PRRSV- and PCV2-1oads in both serum and different tissue samples from co-infected group were significantly higher than those of PRRSV or PCV2 singly infected group. Antibodies to PRRSV and PCV2 developed later and reached significantly lower levels in serum from PRRSV/PCV2 group than from PRRSV or PCV2 group. The proliferation activities of PBLC in three virus-infected groups were suppressed and the degree of severity was shown as follows： PRRSV/PCV2 group 〉 PRRSV group 〉 PCV2 g that PRRSV and PCV2 co-infection can promote the replication of bot and synergistically suppress the immune responses of the co-infected roup. These results indicate h PRRSV and PCV2 in vivo piglets.

关 键 词：猪繁殖与呼吸综合征病毒 猪圆环病毒2型 共感染 病毒载量 抗体效价 刺激指数 免疫抑制 

分 类 号：S858.285.3[农业科学—临床兽医学]