补骨脂水提液及补骨脂素对体外培养成骨细胞的影响  

Effects of Psoralea corylifolia L.Extracts and Psoralen on Osteoblasts Cultured in vitro

作　　者：安亚兰[1] 王建舫[1] 许水明 韩博[1] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]江西省抚州市临川区农业局,抚州344100

出　　处：《畜牧兽医学报》2009年第2期266-271,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(303710653057136230771594)

摘　　要：为了探讨补骨脂水提液及补骨脂素对体外培养成骨细胞的影响，采用MTT法、PNPP（对硝基苯磷酸二钠盐）法及流式细胞术，分别榆测不同浓度的补骨脂水提液（10^-4～10^1mol·L^-1）及补骨脂素（10^-8～10^-4 mol·L^-1）对体外培养小鼠成骨细胞增殖、分化及细胞周期的影响。结果表明，10、10^-3mg·mL^-1补骨脂水提液对成骨细胞有明显的促增殖作用；高浓度（10^-4、10mol·L^-1）补骨脂素抑制细胞增殖，而低浓度（10^-7、10^-8mol·L^-1）补骨脂素能够促进增殖；10^-4、10^-6、10^-8mol·L^-1补骨脂素均能使成骨细胞碱性磷酸酶（ALP）活性明显上升；各浓度的补骨脂水提液及补骨脂素使细胞G，期百分比降低，而S期、G2期百分比显著增加。试验结果证实补骨脂水提液及补骨脂素具有植物雌激素样作用，可以促进成骨细胞增殖，促使细胞由G1期向S期、G2期转化；补骨脂素是促进成骨细胞分化的有效成分。In order to evaluate the effect of Psoralea corylifolia L. extracts and Psoralen with different concentrations on osteoblasts cultured in vitro. The proliferation and the activity of alkaline phosphatase（ALP）were measured by MTT（tetrazolium salt test） and PNPP（P-nitrophenyl phosphate disodium）, respectively, and the cell cycle analysis was examined with flow cytometer by PI（propidium iodide） staining. The results indicated that 10 and 10^-3 mg·mL^-1 of Psoralea corylifolia L. extracts could remarkablely promote osteoblasts proliferation. Psoralen at lower concentrations（10^-7,10^-8 mol ·L^-1） could promote proliferation of osteoblasts, while it could inhibit proliferation at higher concentrations（10^-4,10^-5 mol·L^-1）. ALP activity was significantly increased by different concentrations of Psoralen. The percentage of G1 phase cells was decreased,while the S and the G2 phase were increased. It is suggested that Psoralea corylifolia L. extracts and Psoralen, which promote the proliferation of osteoblasts with a pbytoestrogenic effect, can also induce the transformation from the G1 phase to either the S phase or the G2 phase. Psoralen may play an important role in differentiation of osteoblasts.

关 键 词：补骨脂 补骨脂素 成骨细胞 MTT PNPP 细胞周期 

分 类 号：S852.16[农业科学—基础兽医学]