胰岛素强化治疗对新诊断2型糖尿病患者外周血单核细胞中核因子κB表达的影响  

Effect of intensive insulin therapy on nuclear factor-kB expression in peripheral blood mononuclear cell in newlydiagnosed type 2 diabetics

作　　者：何媛媛[1] 林杨[1] 李素梅[1] 任安[1] 叶山东[1] 陈若平[1] 陈超[1] 荆春艳[1] 翟志敏[2] 

机构地区：[1]安徽医科大学附属安徽省立医院内分泌科,合肥230001 [2]安徽医科大学附属安徽省立医院中心实验室

出　　处：《中国糖尿病杂志》2009年第2期125-127,共3页Chinese Journal of Diabetes

基　　金：安徽省卫生厅临床医学应用技术资助项目(06B032);安徽省科学技术厅重点科研资助项目(06023061)

摘　　要：目的观察胰岛素强化治疗前后初发2型糖尿病(T2DM)患者外周血单核细胞中核因子κB(NF-κB)表达水平的变化,探讨胰岛素的抗炎作用机判。方法健康成人20例为正常对照(NC)组,新诊断T2DM 20例为DM组,DM组胰岛素强化治疗2周。治疗前后采用ELISA、FCM法检测NF-κB表达水平,同时测定治疗前后WBC、N%和FIns,计算HOMA-IR。结果与NC组比,DM组NF-κB表达水平显著增高(P<0.01);DM组与治疗前比,治疗后NF-κB水平显著降低(P<0.05),WBC、N%和HOMA-IR也明显降低(P均<0.01)。结论 2型糖尿病患者体内存在低度炎症,胰岛素强化治疗可降低单核细胞NF-κB表达,减轻炎症反应。Objective To investigate the effect of intensive insuIin therapy on the expression level of nuclear factor-kB in peripheral blood mononuclear cell （PBMC） in newly-diagnosed type 2 diabetics. Methods Twenty normal individuals and 20 newly diagnosed type 2 diabetics were selected. All diabetic patients were given intensive insulin therapy for two weeks. NF-kB expression in peripheral blood mononuclear cell was measured by enzyme-linked immunosorbent assay （ELISA） and flow cytometry （FCM） before and after insulin treatment. The changes of FBG,while blood cell count （WBC）, percent of neutrophilic granulocyte（N%） and fasting serum insulin（Fins） were assayed. Results The expression level of nuclear factor-kB detected by ELISA and FCM in type 2 diabetes patients was significantly higher than that in control subjects （P〈0.01）. The expression level of nuclear factor-kB after treatment was significantly decreased （P〈0. 05）, and the levels of WBC, N% and HOMA-IR were also decreased after insulin intensive therapy （all P 〈 0. 01 ）. Conclusions There may be a low-degree of inflammation reaction in type 2 diabetics. Intensive insulin therapy can decrease the level of NF-kB in PBMC and inflammatory reaction.

关 键 词：2型糖尿病 胰岛素 核因子KB 临床分析 

分 类 号：R587.1[医药卫生—内分泌]