罗格列酮对糖尿病大鼠肾脏PEDF和TGF-β_1表达的影响  

The effect of rosiglitazone on the expressions of PEDF and TGF-β_1 in diabetic rat kidney

作　　者：王战建[1] 刘珊[1] 苏杰英[1] 宋庆芳[1] 庞建华[1] 周亚茹[1] 

机构地区：[1]河北医科大学第三医院内分泌科,石家庄050051

出　　处：《中国糖尿病杂志》2009年第2期143-146,共4页Chinese Journal of Diabetes

基　　金：河北省卫生厅基金资助项目(编号06211)

摘　　要：目的探讨罗格列酮(RGZ)对DM大鼠肾组织色素上皮衍生因子(PEDF)和转化生长因子β_1(TGF-β_1)的影响。方法 SD大鼠随机分为NC组、DM组和RGZ干预组(RGZ组)。12周后,观察各组FBG、肾重、肾重/体重指数、血脂,24小时UAlb的变化,以及肾脏组织标本行免疫组化和Westernblot观察PEDF和TGF-β_1的表达。结果 (1)RGZ组大鼠FBG、肾重、肾重/体重、24小时UAlb、Cr,BUN均低于DM组。(2)免疫组化及Western blot显示,DM组和RGZ组肾组织TGF-β_1表达均高于NC组(P<0.01,P<0.05),而RGZ组TGF-β_1低于DM组(P<0.01);DM组和RGZ组PEDF均低于NC组(P<0.01,P<0.05),而RGZ组PEDF高于DM组(P<0.05)。结论 RGZ通过减低DM大鼠肾脏TGF-β_1和升高PEDF的表达,对肾脏起到保护作用。Objective To investigate the effects of rosiglitazone on the expression of pigment epithelium-derived factor（PEDF） and transforming growth factor-β1 （TGF-β1） in the kidney of diabetic rats. Methods 42 male SD rats were randomly assigned into three groups （n= 14 for each group）： normal group（NC）, diabetic group （DM） and rosiglitazone-treated group （RGZ）. The rats in RGZ group were given rosiglitazone sodium, while the other two groups were given normal sodium in the same volume. Fasting blood glucose, kindney mass, kindney/body mass ratio and urinary albumin excretion （UAE） were measured after 12 weeks of treatment. Serum TG, TC, LDL-C, VLDL-C, HDL-C, BUN and Cr were observed after 12 weeks. The protein expression and location of PEDF and TGF-β1 were obsreved by immunohistochemistry. Moreover, PEDF and TGF-β1 expressions in renal cortex were analyzed by Western blot. Results Compared with DM group, RGZ group showed a significant decrease in kidney mass/body mass ratio, urinary albumin excretion, serum creatinine（Cr）, blood urea nitrogen （BUN）. Compared with NC group, the expression of TGF-β1 was markedly increased in DM group （P〈0.01）. Compared with DM group there was a significant decrease in expression of TGF-β1 in RGZ group （P〈0.05）. The expression of PEDF was markedly decreased in DM versus NC group （P〈 0. 01）, and there was a significant increase in the expression of PEDF in RGZ versus DM group（P〈 0.05）. Conclusions Renoprotection of rosiglitazone in diabetic rats may be mediated through decreasing the expression of TGF-β1 and increasing the expression of PEDF.

关 键 词：糖尿病肾病 色素上皮衍生因子 转化生长因子Β1 罗格列酮 

分 类 号：R587.1[医药卫生—内分泌]