固相萃取-高效液相色谱法测定曲咪新乳膏中硝酸咪康唑和醋酸曲安奈德的含量  

SPE-HPLC determination of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide acetate and miconazole nitrate neomycin sulfate cream

作　　者：黄静[1] 张毅[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《药物分析杂志》2009年第2期305-307,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用C18固相萃取柱分离除去曲咪新乳膏中的基质干扰,高效液相色谱法同时测定硝酸咪康唑和醋酸曲安奈德的含量。方法:色谱柱为Waters Xterra C18(5μm,4.6 mm×250 mm);流动相为乙腈-0.6%醋酸铵溶液(65∶35);检测波长为235 nm;流速为1.0 mL·min-1;柱温为35℃。结果:硝酸咪康唑进样量在0.0998～2.494μg,醋酸曲安奈德进样量在0.0102～0.256μg范围内,线性关系良好。平均回收率硝酸咪康唑为99.5%,醋酸曲安奈德为99.2%。结论:此方法灵敏快速、结果准确、重现性好,可用于该制剂的质量控制。Objective： To establish a SPE - HPLC determination of triamcinolone acetonide acetate and miconazole nitrate in triamcinolone acetonide acetate and miconazole nitrate neomycin sulfate cream. Method：The sample was purified with C18 solid phase column and its content was determined with HPLC method. The Waters Xterra C18 column（5 μm,4.6 mm ×250 mm） was adopted and mobile phase consisted of acetonitrile -0.5% acetate solution （ 65： 35 ）. The UV detector wavelength was at 235 nm. The flow rate was 1.0 mL · min^-1. The column temperature ： 35℃. Results：The linear range of miconazole nitrate was 0. 0998 -2. 494 μg and the average recovery was 99. 5% ;and triamcinolone acetonide acetate was 0. 0102 -0. 256 μg and the average recovery was 99. 2%. Conclusion：The method is simple, quick, sensitive, accurate, reproducible and be adopted to control the quality of tri- amcinolone aeetonide acetate and miconazole nitrate neomycin sulfate cream

关 键 词：固相萃取 硝酸咪康唑 醋酸曲安奈德 

分 类 号：R917[医药卫生—药物分析学]