HPLC法测定螃蟹甲中山栀苷甲酯的含量  

HPLC determination of shanzhiside methyl ester in Phlomis younghusbandii Mukerjee

作　　者：赵斌[1,2] 董小萍[1] 吴健明[1] 

机构地区：[1]成都中医药大学药学院,成都610075 [2]中山火炬职业技术学院,中山528436

出　　处：《药物分析杂志》2009年第2期323-325,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用反相高效液相色谱法测定螃蟹甲药材中山栀苷甲酯的含量。方法:采用TIANHE C18色谱柱(200 mm×4.6mm,5μm),乙腈-水(12∶88)为流动相;流速1.0 mL·min-1;检测波长为236 nm。结果:山栀苷甲酯进样量在0.052～0.52μg范围内,与峰面积呈良好线性关系(r=0.9993);高、中、低3个剂量组平均回收率分别为99.5%,100.6%,98.1%;RSD分别为1.1%,1.3%,0.42%。结论:该方法简便、准确,重复性好,可作为螃蟹甲药材的质量控制方法。Objective： To develop an RP - HPLC method for the determination of shanzhiside methyl ester in Phlomis younghusbandii Mukerjee. Methods：TIANHE C18 column（200 mm × 4. 6 mm ,5 μm）was used with acetonitrile -water （12： 88） as the mobile phase at a flow rate of 1.0 mL · min^-1, the detective wavelength was set at 236 nm. Results：The linear range of shanzhiside methyl ester was 0. 052 -0. 52 μg（ r = 0. 9993）. In the high, medium, low concentration, the average recoveries of shanzhiside methyl ester were 99.5%, 100. 6%, 98.1% with corre-sponding RSD 1.1%, 1.3% ,0.42%. Conclusion： The method is simple and accurate with good repeatability, which can be used for the quality control of Phlomis younghusbandii Mukerjee.

关 键 词：螃蟹甲 山栀苷甲酯 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]