1例早期HIV感染者体内病毒准种的各基因片段分析  

Analysis of HIV quasispecies according to different gene regions during primary HIV-1 infection

作　　者：李林[1] 隋洪帅[1] 鲍作义[1] 刘永健[1] 刘思扬[1] 庄道民[1] 李韩平[1] 李敬云[1] 

机构地区：[1]军事医学科学院微生物流行病研究所全军艾滋病检测中心病原微生物生物安全国家重点实验室,北京100071

出　　处：《中国艾滋病性病》2009年第1期1-3,10,共4页Chinese Journal of Aids & STD

基　　金：973国家重点基础研究项目(2006CB504200)

摘　　要：目的比较1例早期艾滋病病毒(HIV)感染者体内病毒不同基因片段准种复杂度。方法使用套式聚合酶链反应(Nested-PCR),扩增HIV感染者外周血淋巴细胞中病毒的gag、pol、vif-vpr和env区的部分基因,通过克隆、测序方法观察该感染者体内病毒准种分布情况,比较不同基因片段检测病毒准种的可靠性。结果不同基因片段检测病毒准种结果显示,该早期感染者体内的病毒不同基因片段显示的准种基因离散率不同,其中gag区显示出的病毒准种复杂度最高,反映为该区段病毒准种的基因离散率最高,为0·008;其他各区基因(pol、vif-vpr、env)的离散率依次为0·006、0·001、0·000。结论该HIV感染者体内病毒准种的基因中,gag区基因的突变率较高,提示Gag在机体感染HIV早期承受的免疫压力较高,因此在病毒准种研究中,gag区应该作为重要的基因区域。Objective To investigate heterogeneity of different gene areas of virus at an early phase of primary HIV-1 infection. Methods Nest-PCR was used to amplify the gag, pol, vif-vpr and env regions of HIV DNA integrated in the genome of PBMC from a HIV-infected person at the primary phase. The genes were then cloned into pMD-18T vector and sequenced. Viral heterogeneity was studied through checking quasispecies in the primarily infected individual by analyzing these sequences. Results Quasispecies analysis showed that the gene diversities of viral quasispecies reflected by different gene areas were different. Gene complexity of HIV quasispecies in gag gene area was the highest, which was supported by the gene distances in gag area（0.008）. The distances of other genes （including pol, vif-vpr and env ） among quasispecies were 0.006,0.001 and 0.000,respectively.Conclusions Compared to the other genes,gag gene has the highest diversity at the early phase of HIV infection, indicating that gag protein is under strong immunological pressure. Therefore, in the study of quesispecies at early phase of HIV infection, gag gene should be given high priority.

关 键 词：艾滋病病毒 准种 GAG POL vif-vpr ENV 

分 类 号：R373.9[医药卫生—病原生物学]