HPLC法测定黄柏不同炮制品中小檗碱的含量  

Determination of Berberine in Cortex Phellodendri with Different Processing Methods by HPLC

作　　者：时维静[1] 陈文成[1] 谭志静[1] 

机构地区：[1]安徽科技学院,凤阳233100

出　　处：《中国药事》2009年第2期134-136,共3页Chinese Pharmaceutical Affairs

基　　金：安徽省科技厅科技攻关重点项目资助(编号07010300153);安徽省自然科学研究计划项目资助(编号KJ2008B208);蚌埠市科技局产学研资助项目(2007)

摘　　要：目的考察不同炮制方法对黄柏中小檗碱含量的影响。方法采用高效液相色谱法测定盐酸小檗碱含量。色谱柱ZORBAX SB-C18;流动相为乙腈-0.1%磷酸溶液（50∶50）;流速为1.0mL·min^-1;柱温为室温;检测波长为265nm;进样量为20μL。结果在0.46-4.6μg（r=0.9998,）范围内线性关系良好。结论结果表明黄柏丝的小檗碱含量最高。Objective To observe the influence of different processing method on berberine content in Cortex Phellodendri. Methods To determine the content of hydrochloride berberine by HPLC. The ZORBAX SB-C18 column was used with the mobile phase being acetonitrile-0. 1% phosphoric acid （50 ： 50）, flow rate was 1.0mL · min^-1, the column temperature was set at room, detection wavelength was 265nm, and sampling volume was 20μL. Results The calibration curve was linear over the range of 0.46- 4. 6μg （r=0. 9998） . Conclusions The content of hydrochloride berberine was the highest in the Cortex Phellodendri silk.

关 键 词：黄柏 炮制 提取工艺 HPLC 

分 类 号：R927.2[医药卫生—药学] R284.2