灯盏细辛注射液对TNF-α诱导内皮细胞炎症分子的影响  

Effect of Erigeron breviscapus injection on the expression of inflammatory molecules induced by TNF-α in endothelial cells

作　　者：郭虹[1] 康立源[1] 柴丽娟[1] 胡利民[1] 赵琳[1] 

机构地区：[1]天津中医药大学中医药研究院,天津市中药药理学重点实验室,天津300193

出　　处：《中国新药杂志》2009年第3期253-256,共4页Chinese Journal of New Drugs

基　　金：国家973计划基金(2005CB523404);国家自然科学基金(30572348);云南省科技计划基金(2006YX03)

摘　　要：目的:研究灯盏细辛注射液(Erigeron breviscapusinjection,EBI)对离体培养的大鼠脑微血管内皮细胞(BMEC)炎症相关细胞因子白细胞介素-1β(IL-1β)及细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)蛋白表达的影响,初步探讨EBI抗脑缺血炎症损伤的机制。方法:采用肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)建立离体培养的大鼠BMEC炎症损伤模型,分别用0.1 mg.L-1和1 mg.L-1EBI处理内皮细胞,使用ELISA试剂盒分析IL-1β蛋白表达的变化,流式细胞仪测定ICAM-1和VCAM-1荧光强度的变化。结果:EBI可以抑制TNF-α诱导的内皮细胞IL-1β高表达,降低TNF-α诱导的内皮细胞ICAM-1和VCAM-1高表达。结论:EBI可以降低由TNF-α诱导的内皮细胞过度表达IL-1β,ICAM-1和VCAM-1,从而对脑缺血炎症损伤具有一定保护作用。Objective： To investigate the effect of Erigeron breviscapus injection （EBI） on the expression of interleukin-1β （IL-1β）, intercellular adhesion molecule-1 （ICAM-1）, and vascular cell adhesion molecule-1 （ VCAM-1 ） in rat brain microvascular endothelial cells （BMEC） cultured in vitro. Methods ： The inflammatory injury was induced by tumor necrosis factor-α （TNF-α） in BMECs. The endothelial cells were treated with EBI at 0. 1 and 1 mg·L^-1 The expression of IL-1β protein was detected by ELISA, and those of ICAM-1 and VCAM-1 were measured by flow cytometry. Results ： EBI inhibited TNF-α-enhanced expression of IL-1β in BMECs. Meanwhile it diminished TNF-α-enhanced expression of ICAM-1 and VCAM-1 in BMECs. Conclusion： EBI decreases TNF-α- enhanced expression of IL-1β, ICAM-1 and VCAM-1 in BMECs, resulting in a protective effect on inflammatory injury after cerebral ischemia.

关 键 词：灯盏细辛注射液 脑微血管内皮细胞 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学] R743.31[医药卫生—中医学]