高效液相法测定石杉碱甲在大鼠血浆和脑组织中的浓度  

Determination of huperzine A in rat plasma and brain tissue by HPLC method

作　　者：王俏[1] 陈国神[1] 

机构地区：[1]浙江省医学科学院药物研究所,杭州310013

出　　处：《中国新药杂志》2009年第4期369-371,376,共4页Chinese Journal of New Drugs

基　　金：浙江省卫生厅基金项目资助(2006A003)

摘　　要：目的:建立高效液相色谱法测定大鼠鼻腔给药后血浆和脑组织中石杉碱甲的浓度。方法:色谱柱为岛津Shim-pack CLC-ODS C18柱(150mm×6.0mm,5μm);流动相:甲醇-水(43∶57),其中水相每1000mL中加入三乙胺2mL,用磷酸调pH值至6.9;流速为1.0mL·min-1;检测波长为308nm;柱温为37℃。以石杉碱乙为内标。结果:石杉碱甲血浆浓度在2.5～150ng·mL-1,脑中浓度在2.5～40ng·g-1范围内与峰面积比值有良好的线性关系,平均相对回收率血中和脑中分别为96.6%和95.3%,日内日间精密度<12.1%,定量限为2.5ng·mL-1或ng·g-1。结论:所选用的高效液相色谱法可用于测定石杉碱甲大鼠鼻腔给药后血浆和脑组织中的药物浓度。方法专属性好,精密度和回收率高。Objective：To establish a HPLC method for the determination of huperzine A in rat plasma and brain tissue. Methods：Huperzine A and internal standard,huperzine B were analyzed on a Shim-pack CLC-ODS C18 column （150 mm ×6.0 mm,5 μm） with a mobile phase consisting of methanol-water（43： 57）, in which aqueous phase contained 0. 2% triethylamine and adjusted pH to 6. 9 with phosphoric acid. The flow rate was 1 mL·min^-1 The detection wavelength was at 308 nm. The column temperature was set at 37 ℃. Results：The assay was linear over the concentration range of 2.5 -150 ng·mL^-1 in plasma and 2.5 -40 ng·g^-1 in brain tissue. The intra- and inter-day precisions were below 12. 1%. The average relative recoveries were 96.6% and 95.3% in plasma and brain tissue, respectively. The lower limits of quantification in bio-samples were 2.5 ng·mL^-1 or 2.5 ng·g^-1. Conclusion：The method was successfully applied to determine the huperzine A concentration in rat brain tissue and plasma after nasal administration. The method was accurate, good reproducible and high recovery.

关 键 词：高效液相色谱法 石杉碱甲 石杉碱乙 脑 血浆 鼻腔吸收 

分 类 号：R969.11[医药卫生—药理学] R971[医药卫生—药学]