细胞DNA指数全自动分析仪及其关键技术  

Cell DNA index autoanalyzer and its key technologies

作　　者：鲁劲松[1,2] 段侪杰[1] 俞燕明[1] 张宏印[2,1] 张艺宝[1] 艾华[1] 侯凯[3] 包尚联[1,2,3] 

机构地区：[1]北京大学医学物理和工程北京市重点实验室,北京100871 [2]北京大学肿瘤物理诊疗技术研究中心北京大学医院,北京100871 [3]北京海思威科技有限公司,北京100084

出　　处：《仪器仪表学报》2009年第2期293-297,共5页Chinese Journal of Scientific Instrument

基　　金：973项目(2006CB705705);国家自然科学基金(10527003;60672104);北京教委共建项目(SYS100010401);北京自然科学基金(3073019);科技部仪器升级改造项目(2006JG1000);国防重点项目;博士点基金(20040001003)资助项目

摘　　要：设计了测量细胞DNA指数的全自动分析仪(HW-MS)并发展了相应的关键技术。本仪器用于对细胞涂片的数字化显微扫描,获得细胞DNA指数的分布数据。HW-MS采用了排除外界干扰和对测量结果进行归一化的技术措施,使得同一涂片的测量结果具有较好的稳定性。HW-MS的功能包括对涂片自动扫描,实现对细胞图像的自动采集,然后对存贮在计算机内细胞图像进行降噪和分割处理,并对处理后的图像进行识别和分类分析,实现对细胞DNA指数的测量,最后对测量结果中突变细胞进行识别和统计分类。为了保证测量数据的准确性,系统使用标准样片来实现图像质量控制。在高速模式下,本系统可以在30min内实现全片的自动扫描,基本上同时给出测量样本的细胞DNA指数分布数据。初步实验表明,该系统的测量结果具有较高的准确性和稳定性。A cell DNA index autoanalyzer （HW-MS） was designed and its key technologies were developed. The analyzer can automatically scan a sample glass plate and acquire the digital cell image, which is used in DNA index measurement. The HW-MS adopts the technology that can suppress the disturbance from environment during the scanning, and proper algorithm to normalize the images. The functions of the device include auto-scanning cell glass plate to realize digital cell data acquisition; storing cell image data on hard disk, further data processing such as noise reduction and imaging segmentation; and cognizing and classifying the mutation cells. In order to ensure the accuracy of the measurement results, the autoanalyzer uses standard sample plate to realize image quality control. HW-MS can scan a standard size glass plate within 30 minutes, and the distribution of the cell DNA index is obtained immediately. Experiment results show that the autoanalyzer has good reliability, accuracy and stability.

关 键 词：自动显微图像处理 DNA指数 图像分析 

分 类 号：TP216[自动化与计算机技术—检测技术与自动化装置]