川芎嗪对自体移植内皮祖细胞修复兔受损腹主动脉内膜的影响  

Influence of Ligustrazine on Transplantation of Autologous Endothelial Progenitor Cells in Repairing Endangium of Injured Abdominal Aorta in Rabbits

作　　者：刘俊松[1] 马根山[1] 姚玉宇[1] 胡耀鹏[1] 沈成兴[1] 张晓黎[1] 

机构地区：[1]东南大学临床医学院,江苏南京210009

出　　处：《中国微循环》2009年第1期15-18,73,共5页Journal of Chinese Microcirculation

摘　　要：目的通过在体实验研究川芎嗪对内皮祖细胞(EPCs)修复球囊损伤后的兔腹主动脉的影响,探讨更好的损伤性血管修复的方法。方法密度梯度离心法分离兔外周血单个核细胞,以EBM-2培养基培养7d,获得兔外周血内皮祖细胞,应用2.5F球囊导管造成兔腹主动脉去内皮损伤模型。将实验兔分为三组,每组6只,对照组使用生理盐水,EPCs组只使用EPCs,EPCs+川芎嗪组注射EPCs及川芎嗪,术后14d处死动物,并测量各组内膜损伤血管再生内皮覆盖率和新生内膜增生程度。结果EPCs+川芎嗪组新生内膜/中膜厚度明显低于EPCs组,新生内膜面积明显高于EPCs组,两组均与对照组相比差异有统计学意义。结论EPCs可促进受损血管内膜修复,川芎嗪具有增强EPCs修复血管内膜的作用。Objective To study the influence of ligustrazine on transplantation of autologous endothelial progenitor cells in repairing endangium of abdominal aorta in rabbits after balloon injury, and find a better method to repair injured endangium. Methods Mononuclear cells （MNC） were isolated from rabbit peripheral blood by density - gradient centrifugation. MNC were cultured in endothelial growth medium for 7 days, yielding EPC. Saline alone （ control group）, autologous EPC and autologous EPC plus ligustrazine were administered into a rabbit model of balloon abdominal aorta injury. 14 days after transplantation, rabbits were killed. Endothelial regeneration rate and interal area/media area （IA/MA） ratio were detected. Results 14 days after transplantation, autologous EPC group and autologous EPC plus ligustrazine group compared with saline admin- istration markedly accelerated endothelialization of denuded vessel, especially in autologous EPC plus ligustrazine group. Interal area/media area （IA/MA） ratio of autologous EPC plus ligustrazine group is smaller than autologous EPC group significantly, and interal area/media area （IA/MA） ratio of autologous EPC group is smaller than autologous EPC plus ligustrazine group significantly. Conclusion Transplantation of EPC to balloon - injured arteries is associated with accelerated reendothelialization and ligustrazine can enhance the effect of EPCs.

关 键 词：川芎嗪 内皮祖细胞 血管 内膜 增生 

分 类 号：Q25[生物学—细胞生物学]