培养条件对栗疫菌毒素产生的影响  

The Effects of Cultural Conditions on Production of Cryphonectria parasitica Phytotoxin

作　　者：韩珊[1] 朱天辉[1] 李芳莲[1] 

机构地区：[1]四川农业大学林学园艺学院,四川雅安625014

出　　处：《四川林业科技》2009年第1期66-70,共5页Journal of Sichuan Forestry Science and Technology

基　　金：国家自然科技资源共享平台基金(2005DKA21207-13);四川省学术和技术带头人培养基金资助

摘　　要：本文以中国板栗当年生幼嫩枝条作为栗疫菌粗毒素生物检测材料,对该病菌的产毒条件进行了研究。结果表明:栗疫菌在不同培养液的产毒能力有明显的差异,7种培养液中C(PD+板栗煎汁)培养液产毒能力最强;栗疫病菌产毒能力以培养温度为26℃,培养基pH值为6的条件下培养18 d生物活性最强;最佳氮源为蛋白胨;较好的碳源为糊精。Young branches of Chinese chestnut were selected to test the toxicity of crude toxin by Cryphonectria parasitica, aiming to study of the conditions of phytotoxin-producity capability. The results showed that C liquid medium had the strongest phytotoxin in 7 media. The optimal cultural conditions for producing toxin were as follows ：the temperature was 26℃ ,the cultured period lasted 18 d ,and pH value was 6. Peptone was the best nitrogenous source. Dextrin was better among the selected carbonic sources.

关 键 词：板栗疫菌 真菌毒素 培养条件 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]