益气活血中药对放射性肺损伤大鼠血清ET-1和TNF-α的影响  

Effect of Yiqi Huoxue Recipe on the Expression of ET-1 and TNF-α in Rats with Radiation-induced Lung Injury

作　　者：李凤玉[1] 王炳胜[1] 刘秀芳[1] 张海[1] 左宏[1] 石军[1] 张建宇[1] 葛艳丽[1] 任成波[1] 

机构地区：[1]解放军251医院肿瘤诊疗中心,河北张家口075000

出　　处：《华北国防医药》2009年第1期6-8,共3页Medical Journal of Beijing Military Region

基　　金：全军"十一五"课题基金资助项目(No.06MB084)

摘　　要：目的:观察小剂量重复照射下不同时相大鼠血清内皮素(ET-1)和肿瘤坏死因子-α(TNF-α)的改变并探讨益气活血中药对放射性肺损伤的防治作用。方法:将96只W istar雌性大鼠随机分为照射加中药组(A组)30只、照射加氨溴索组(B组)30只、单纯照射组(C组)30只、正常对照组(D组)6只。用直线加速器对前三组大鼠右肺进行分次照射(5 Gy/次,1次/周,累积剂量最高为30 Gy),于照射开始后第4、6、8、12、26周5个时间点分别处死A、B、C组各6只大鼠,取血清标本进行ELISA检测,观察各组大鼠不同时相血清ET-1和TNF-α的动态变化。结果:3组血清ET-1水平于照射后4周进行性升高,8周达高峰,12周开始下降;3组血清TNF-α水平4周进行性升高,12周达高峰,26周开始下降。A组各时相大鼠血清ET-1、TNF-α表达水平均低于C组(P<0.05),且各时相值与B组近似,差异无统计学意义(P>0.05)。结论:血清ET-1、TNF-α水平的变化能反映早期放射性肺损伤的严重程度,益气活血中药能够降低血清ET-1、TNF-α的水平,可能是其防治放射性肺损伤的作用机制。Objective：To explore the changes of ET-1 ,TNF-α in plasma and the curative effect of Yiqi Huoxue Recipe in rats with radiation-induced lung injury in different radiation times. Methods：96 W istar rats were randomly divided into irradiation group：group A（ n = 30 ） irradiation with Yiqi Huoxue Recipe group ; group B （ n = 30） irradiation with ambroxol group ; group C （ n = 30） irradiation group and group D （n = 6） normal control group. The first three groups were irradiated at right hemithorax with a dose of 30 Gy/6 Fraction/5 weeks. Animals were followed for 26 weeks after the first irradiation. The levels of serum ET-1 and TNF-α of 5 rats in each group were assayed by ELISA at the end of the 4th,6th,8th,12th and 26th week after the first irradiation. Results：The levels of serum ET-1 and TNF-α of the 3 groups increased progressively in the 4th week after irradiation, reached the peak in the 8th week and began to drop in the 12th week, but TNF-α level reached the peak in the 12th week and began to drop in the 26th week. The expression level of ET-1 and TNF-α were significantly lower than that of group C（P 〈0. 05） ,and in ambroxol-group,the levels of serum ET-1 and TNF-α were same to that of the Yiqi Huoxue Recipe group with no statistical significance（ P 〉 0.05 ）. Conclusion ： ET-1 and TNF-α can reflect the degree of radiation-induced lung injury. Yiqi Huoxue Recipe can decrease the levels of ET-1 and TNF-α in plasma significantly. This might be one of the mechanisms in the treatment and prevention of radiation-induced lung injury.

关 键 词：中草药 益气活血 辐射性肺损伤 大鼠 内皮素-1 肿瘤坏死因子-α 

分 类 号：R332[医药卫生—人体生理学] R287[医药卫生—基础医学]