巯基葡聚糖凝胶分离富集二溴邻硝基偶氮胂分光光度法测定汞(Ⅱ)  

Determination for Mercury(Ⅱ) by Dibromo-o-nitro-arsenazo Spectrophotometry with Separation and Concentration of Sulphydryl Dextran Gel

作　　者：张秋荣[1] 陈文宾[1] 陈碧珠[1] 林艳[1] 许兴友[1] 马卫兴[1] 

机构地区：[1]淮海工学院化学工程系,江苏连云港222005

出　　处：《环境监测管理与技术》2009年第1期18-21,共4页The Administration and Technique of Environmental Monitoring

基　　金：教育部重点实验室开放基金资助项目(CPEUKF06-04);江苏省海洋生物技术重点建设实验室基金资助项目(2005HS001;2005HS010;2006HS020);江苏省高等学校大学生实践创新训练计划基金资助项目(2008603);淮海工学院自然科学基金资助项目(Z2006001)

摘　　要：研究了溴代十六烷基吡啶、正丁醇、正庚烷和水自制微乳溶液介质中,汞(Ⅱ)与二溴邻硝基偶氮胂的显色反应,采用巯基葡聚糖凝胶分离共存离子并富集汞(Ⅱ),建立了分光光度法测定微量汞(Ⅱ)的新方法。在pH值为6.00的乙酸-乙酸钠缓冲溶液中,汞(Ⅱ)与试剂形成紫红色络合物,其最大吸收峰位于530 nm波长处,表观摩尔吸光系数ε为3.3×105L/(mol.cm)。在显色液中汞(Ⅱ)质量浓度在8.00μg/L^600μg/L范围内符合比尔定律,检出限为0.003 mg/L,水、牛奶、化妆品测定的RSD为1.4%~6.1%,加标回收率为96.8%~105%。The color reaction of Dibromo-o-nitro-arsenazo with Hg（ Ⅱ ） was studied in the solution of micromulsion CPB, n-C4H9OH, C7 H 14 and H2O. A new method was established for trace Hg （ Ⅱ ） determination by spectrophotometry with separation of co-ions and concentration of Sulphydryl Dextran Gel. In the pH 6.00 buffer of HAc-NaAc, the reagent reacted with Hg（ Ⅱ ） to form a purple complex. The maximum absorption wavelength located at 530 nm and apparent molar absorptivity ε was 3.3 × 10^5 L/（mol · cm）. In the colorful solution Beer＇s law was obeyed in the range of 8.00 μg/L- 600 μg/L for Hg（ Ⅱ ）and the detection limit was 0. 003 mg/L. The sample RSDs of water,milk,consmetics were 1.4%- 6.1% , and recoveries were from 96.8% to 105%.

关 键 词：汞(Ⅱ) 巯基葡聚糖凝胶 二溴邻硝基偶氮胂 分光光度法 

分 类 号：O657.32[理学—分析化学]