柿叶黄酮预处理对大鼠离体心脏缺血再灌注损伤的保护作用  

Protective Effect of Persimmon Flavone Pretreatment on Rat Myocardial Ischemic and Reperfusion Injury

作　　者：孙懿[1] 谭宏棣[1] 蓝晓步[1] 黄建春[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室

出　　处：《济宁医学院学报》2009年第1期9-11,共3页Journal of Jining Medical University

基　　金：广西壮族自治区科学基金项目(桂科0832013Z)

摘　　要：目的观察柿叶黄酮预处理对大鼠离体心脏缺血再灌注损伤的保护作用。方法48只Wistar雄性大鼠随机分为正常对照组,心肌缺血再灌注组,柿叶黄酮高、中、低剂量组,阳性药对照组。离体大鼠心脏采用Langendorff法灌流,停灌30min再灌30min造成心肌缺血再灌注损伤模型。观察柿叶黄酮各组对冠脉流出液中肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、乳酸脱氢酶同工酶-1活性以及心肌组织中超氧化物歧化酶、Na+-K+-ATP酶、Ca2+-ATP酶活性和丙二醛含量的影响。结果柿叶黄酮高、中剂量均可显著改善缺血-再灌注所致的心功能损伤,减少肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、乳酸脱氢酶同工酶-1的释放和心肌组织丙二醛的产生,增加超氧化物歧化酶、Na+-K+-ATP酶和Ca2+-ATP酶的活性(P<0.01或P<0.05)。结论柿叶黄酮对心肌缺血-再灌注损伤具有保护作用,其机制可能与提高超氧化物歧化酶活性、抗氧自由基、减少脂质过氧化反应有关。Objective To observe the protective effect of Persimmon flavone（PF） pretreatment on rat myocardial ischemic and reperfusion （I/R） injury. Methods 48 Wistar male rats were randomly divided into control, I/ R group, high - , medium- and low -dose PF groups, and verapamil （Ver） group. The isolated perfused rat hearts were set up by Langendorff- perfusion system. Myocardial ischemia reperfusion injury was induced by 30 minutes global ischemia followed by 30 minutes reperfusion. The activities of creatine kinase （CK）, creatine kinase isoenzyme MB （ CK - MB）, lactate dehydrogenase （ LDH）, lactic dehydrogenase （ LDH - 1 ） in the effluent from aeteria coronaria were measured to evaluate the injury of myocardial cells and the protective effect of PF. Meanwhile, the activities of superoxide dismutase （SOD）, Na ^＋ - K ^＋ - ATPase and Ca^2＋ - ATPase, and the content of malondialdehyde （MDA） in myocardial tissues were determined. Results High - and medium - dose PF could obviously recover cardiac function, reduce the releases of CK, CK - MB, LDH, LDH - 1 from I/R rat hearts, increase the activities of SOD, Na ^＋ - K ^＋ - ATPase and Ca^2＋ - ATPase, and decrease the MDA product （P 〈 0.01 or P 〈 0.05）. Conclusion PF may offer myocardial protective effect against I/R injury. The mechanism may be related to attenuating free radicals.

关 键 词：柿叶黄酮 缺血再灌注 自由基 肌酸激酶同工酶 

分 类 号：R977.3[医药卫生—药品] R972[医药卫生—药学]