大鼠脊髓损伤后对红核神经元逆行性损伤的实验研究  

Experimental Study on Retrograde Trauma of Red Nucleus Neurons after Spinal Cord Injury of in Rats

作　　者：孔令胜[1] 于如同[2] 聂冬丽[1] 陈德勤[1] 赵万巨[1] 

机构地区：[1]济宁医学院附属医院 [2]徐州医学院附属医院

出　　处：《济宁医学院学报》2009年第1期12-14,共3页Journal of Jining Medical University

基　　金：江苏省自然科学基金资助项目(BK2003025)

摘　　要：目的研究大鼠脊髓损伤后对脑红核神经元的逆行性损伤作用。方法采用改良Allen法制备大鼠SCI模型,伤后28d采用辣根过氧化酶逆行示踪技术标记RN神经元,应用四甲基联苯胺呈色反应显示红核脊髓束神经元的存活情况。结果SCI后28dHRP标记RN神经元胞体模糊,排列零散,HRP标记RN神经元数目与假手术组比较显著减少(P<0.001),差异有统计学意义。结论大鼠SCI后对脑RN神经元和RST具有逆行性的损伤作用,这在SCI后神经功能恢复的形态学研究中起着重要作用。Objective To study on retrograde trauma of red nucleus neurons after spinal cord injury（SCI） in rats. Methods SCI model of Sprague Dawley rats was induced with modified Allen＇s method, 28thd after SCI, rubrospinal tract （RST） neurons were labeled by retrograde transport of the horseradish peroxidase （HRP） from the lesion site, which were taken by damaged axons and remained in the neurons, then the labeled red nucleus （RN） neurons were counted. Results After 28thd, the cell body of RST neurons labeled by HRP in SCI group was illegible and scattered, the number of RST neurons labeled by HRP in SCI group was more than in Sham group, there were significant differences between two groups （ P 〈 0. 001 ）. Conclusion Conclusions There was a retrograde trauma of RN and RST after SCI in rats, it played an important role in morphological research of functional recovery after SCI.

关 键 词：SCI RN 逆行性损伤 

分 类 号：R651.2[医药卫生—外科学] R816.5[医药卫生—临床医学]