甲壳低聚糖-硒对THP-1源性巨噬细胞抗氧化酶相关基因表达的影响  

Effect of chitooligosaccharides-selenium on expression of antioxidant enzyme related genes in THP-1 derived macrophages

作　　者：李洁[1,2] 黄春林[1] 曹运长[1] 李邦良[1] 

机构地区：[1]南华大学生化与分子生物学教研室,湖南衡阳421001 [2]南华大学机能学实验中心,湖南衡阳421001

出　　处：《中国生化药物杂志》2009年第1期24-26,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：湖南省衡阳市科技局研究项目(2007KJ022)

摘　　要：目的初步探讨甲壳低聚糖-硒(COS-Se)抗氧化的可能分子机制。方法用100μg/mL的COS-Se处理THP-1源性巨噬细胞6,12,24,48 h,运用半定量RT-PCR方法检测其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)mRNA表达水平的变化。结果COS-Se处理THP-1源性巨噬细胞24和48 h后,SOD mRNA的表达与对照组比较分别上调了14.4%,16.9%(P<0.05);处理6,12,24,48 h后,GSH-Px mRNA的表达与对照组比较分别上调了12.7%,10.9%,13.1%(P<0.05)和27.3%(P<0.01)。结论COS-Se具有抗氧化功能可能与其能上调巨噬细胞中SOD、GSH-Px mRNA表达水平有关。Purpose To study the antioxidant mechanism of chitooligosaccharides-selenium （COS-Se）. Methods THP-l-derived maerophages were cultured and treated with COS-Se （100 μg/mL） for 6, 12, 24 and 48 h respectively. Then SOD, GSH-Px mRNA expression was determined by semi-quantity RT-PCR. Restilts Compared with control, COS-Se significantly increased expression of SOD mRNA after 24 h and 48 h and GSH-Px mRNA expression after 6, 12, 24 and 48 h. Conclusion COS-Se could up-regulated SOD and GSH-Px mRNA expression in macrophage cells in vitro, which could be related with the antioxidant function possessed by COS-Se.

关 键 词：甲壳低聚糖-硒 THP-1细胞 抗氧化 基因表达 

分 类 号：Q78[生物学—分子生物学] R285.5[医药卫生—中药学]