玉米基因sbe1 cDNA的克隆与原核表达  

CLONE AND EXPRESSION OF sbe1 cDNA FROM MAIZE IN E.coil

作　　者：张军杰[1,2] 周会[1] 胡育峰[1] 田孟良[1,3] 刘汉梅[1,2] 黄玉碧[1,3] 

机构地区：[1]四川农业大学玉米研究所,四川雅安625014 [2]四川农业大学生命理学院,四川雅安625014 [3]四川农业大学农学院,四川雅安625014

出　　处：《核农学报》2009年第1期70-74,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家863项目(2008AA10Z123);教育部长江学者和创新团队发展计划资助(IRT0453);四川省教育厅青年基金项目(2006B010)

摘　　要：采用RT-PCR技术克隆了玉米基因sbe1全长cDNA序列,在NCBI上序列比对后与文献报道的sbe1基因序列同源性达到99%。同时构建了原核生物表达载体pET32(a+)-sbe1,成功表达出了SBEⅠ蛋白。为进一步体外研究SBEⅠ的物理化学性质及催化机理奠定基础。The cDNA of sbe 1 c from maize was cloned by RT-PCR and compared with the reported sequence of she 1 cDNA in GenBank.The results showed that the nuleotide sequence homology were 99%. The sbel cDNA were inserted pET32a（ ＋ ） vector by restriction endonuclease digestion, electrophoresis, gel reclamation DNA ligation and so on molecular biological technologies, and that which were transformed to E. coil BL21 （DE3）. At the same time sbel cDNA was induced expression in E. coil BL21（DE3） by IPTG, and the SBE Ⅰ protein was expressed. The experimental result lay a foundation for further study on the physical and chemical properties of SBE Ⅰ in vitro.

关 键 词：玉米 sbe1 基因 基因表达 

分 类 号：S513[农业科学—作物学]