胎衣不下奶牛胎盘中TNF-α和IL-10含量变化的研究  

Alterations of the TNF-α and IL-10 Content in the Placenta of Cows with Retention of Fetal Membrane

作　　者：陈斌[1] 周帮会[2] 韩旭东[1] 邵宾[1] 黄会岭[1] 姜国均[2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]河北农业大学中兽医学院,河北定州073000

出　　处：《安徽农业科学》2009年第4期1553-1553,1559,共2页Journal of Anhui Agricultural Sciences

基　　金：河北省畜牧兽医局科技项目(200606)

摘　　要：[目的]通过研究胎衣不下奶牛胎盘组织中TNF-α和IL-10含量的变化,探讨奶牛发生胎衣不下的免疫学机制。[方法]采集健康奶牛和胎衣不下奶牛的胎儿胎盘,制备胎盘组织匀浆液,用酶联免疫吸附法(ELISA)检测其中的TNF-α和IL-10含量。[结果]胎衣不下组奶牛胎盘组织中的TNF-α含量高于健康组奶牛,差异极显著(P<0.01);IL-10含量低于健康组奶牛,差异显著(P<0.05)。[结论]胎盘组织中TNF-α和IL-10的含量与奶牛胎衣不下的发生有着密切关系。[ Objective ] Through the investigate the fluctuating regulation of placenta TNF-α and IL-10 cytokines plane in cows with retention of fetal membrane （RFM）, this study aimed to search for the immunological mechanisms of RFM. [ Method] Collect the foetal placenta of cows with RFM and healthy cows. The content of TNF-α and IL-10 in foetal placenta supernatant were measured by Enzyme Linked Immunosorbent Assay （ELISA）. [ Results] The level of TNF-α of RFM group was significantly higher than that of the healthy group（ P 〈 0. 01 ） ; and the level of IL-10 of RFM group was higher than that of healthy group（P 〈 0.05 ） . [ Conclusion ] RFM was associated with the level of TNF-α and IL-10 in the placenta.

关 键 词：胎衣不下 奶牛 胎盘 TNF-Α IL-10 

分 类 号：S823.91[农业科学—畜牧学]