柄海鞘神经复合体的显微观察  

Microscopic Observation of the Neural Complex of Ascidian Styela clava

作　　者：李霞[1] 孙虎山[1] 王宜艳[1] 李华[2] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]滨州学院学工部,山东滨州256600

出　　处：《安徽农业科学》2009年第4期1560-1562,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"973"计划项目(G1999012005)

摘　　要：采用甲苯胺蓝染色和Golgi镀银法对柄海鞘神经复合体的结构进行显微观察,并通过免疫组织化学方法对甲硫氨酸脑啡肽在神经复合体中的分布进行了研究。①甲苯胺蓝染色法显示,神经复合体由神经节、神经腺和疏松结缔组织组成;神经节分为皮质部、髓质部和过渡带;神经腺位于神经节背侧面,由腺小叶组成;神经细胞呈深蓝色,神经纤维呈浅蓝色,与背景反差较大。Golgi镀银法显示的结果不理想,神经细胞和神经纤维着色不明显。②免疫组化结果显示,M-ENK在神经复合体中分布广泛,神经细胞、神经纤维及上皮细胞均有Met-ENK阳性反应。The neural complex of ascidian Styela clara was observed by two histological methods, and the methionine-enkephalin （M-ENK） in the neural complex of Styela clava was analyzed by the immunohistochemical S-ABC （strept avidin-biotin complex） technique, respectively. （1） Toluidine blue staining procedure showed that the neural complex consisted of cerebral ganglion, neural gland and loose connective tissue; the cerebral ganglion was divided into three parts as cerebral cortex, medullary layer and transitional zone; the neural gland was composed of glandular lobules, closely apposing at ventral side of the cerebral ganglion ; the neurons and nerve fibers were colored deeply, making a distinct contrast with the background, while neither the neurons nor the nerve fibers were distinctly colored by Golgi-argentraffin staining procedure. （2） the M-ENK immunoreactive positive ceils and nerve fibers were widely distributed in the neural complex.

关 键 词：柄海鞘 神经复合体 显微结构 甲苯胺蓝染色 甲硫氨酸脑啡肽 

分 类 号：S865.33[农业科学—野生动物驯养]