灯盏花组培快繁与植株再生  

Rapid Propagation and Plant Regeneration of Erigeron breviscapus(Vaniot) Hand.-Mazz.

作　　者：林丽飞[1] 陶发清[2] 胡先奇[3] 金秋[1] 吴丹[1] 白学贵[1] 熊力军[1] 

机构地区：[1]云南红河学院生物系,云南蒙自661100 [2]中国科学院昆明植物研究所,云南昆明650204 [3]云南农业大学云南省植物病理重点实验室,云南昆明650201

出　　处：《安徽农业科学》2009年第4期1580-1581,共2页Journal of Anhui Agricultural Sciences

基　　金：云南省自然科学基金(2003C0044M);红河学院硕士专项课题(XSZ05030)

摘　　要：[目的]建立较为系统的灯盏花组培体系,培育、壮大和发展灯盏花这一独具特色和优势的产业。[方法]以灯盏花(Erigeron bre-viscapus)的叶片为外植体,用不同浓度的激素6-BA、2,4-D、KT和NAA对其进行愈伤组织的诱导和再生植株的研究。[结果]诱导愈伤组织最佳的培养基是MS+KT0.5 mg/L+2,4-D10 mg/L和MS+2,4-D0.5 mg/L+6-BA0.5 mg/L,诱导率为100%;二者相比,前者长出的愈伤组织较为紧密,而后者松散性好;MS+6-BA0.5 mg/L+10%香蕉汁是诱导芽最佳的培养基,达87%;加入0.3 mg/L NAA的1/2MS培养基对生根最有利,生根率达83%。[结论]生长素与细胞分裂素的合理配比有利于快速构建灯盏花培养体系。[ Objective ] To establish a systematic mechanism of Erigeron breviscapus tissue culture for culturing, strengthening and developing an advantageous industry with unique characteristics. [ Method ] The leaves of Erigeron breviscapus were used as explants to induce callus, buds and become to plant regeneration in different optimal medium. [ Result] MS ＋ KT 0.5 mg/L ＋ 2,4-D l0 mg/L and MS ＋ 2,4-D 0.5 mg/L ＋ 6-BA0. 5 mg/L were best to induce callus, comparatively, the latter was looser than the former. The optimal medium for bud differentiation was MS ＋6-BA 0.5 mg/L ＋ 10% banana and differentiation rate was 87%. And that 1/2MS with 0.3 mg/L NAA was suitable for rooting and the rooting rate was 83%. [ Conclusion] Rational ratio of auxin and eytokinin was favorable for quickly establishing a systematic mechanism of Erigeron breviscapus culture.

关 键 词：灯盏花 叶片 组织培养 植株再生 

分 类 号：S336[农业科学—作物遗传育种]