口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域单克隆抗体识别的抗原表位分析  

Analysis of Antigenic Epitopes Recognized by Monoclonal Antibody against Porcine Integrin β6 Subunit LBD as FMDV Receptor

作　　者：赵建勇[1,2] 伏小平[1] 独军政[2] 高闪电[2] 冯艳丽[1] 常惠芸[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州兽医研究所国家口蹄疫参考实验室家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,甘肃兰州730046

出　　处：《安徽农业科学》2009年第4期1583-1585,共3页Journal of Anhui Agricultural Sciences

基　　金：国家高技术研究发展计划(863)资助项目(2006AA10A204);国家支撑计划资助课题(2006BAD06A14)

摘　　要：[目的]分析测定口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域单克隆抗体(mAb)的抗原识别位点、饱和值、亲和力常数。[方法]利用叠加ELISA法、间接ELISA法、硫氰酸盐洗脱法测定C2D8、B7B6、B8C9、C4C7、E7C7 5株mAb的抗原识别位点、饱和值、亲和力常数。[结果]5株mAb间具有相似或不同的抗原识别位点,其中C4C7与E7C7、C4C7与B8C9、E7C7与B8C9等mAb间的识别位点相似,而C4C7与B7B6、E7C7与C2D8等mAb间的识别位点不同。5株mAb C2D8、E7C7、B8C9、C4C7、B7B6的饱和值分别为1∶200、1∶400、1∶800、1∶800、1∶800,亲和力为B8C9>C4C7>B7B6≥E7C7>C2D8。[结论]分析测定了5株mAb的抗原识别位点、饱和值、亲和力常数,从而为利用mAb深入研究整联蛋白αvβ6在口蹄疫病毒感染过程中的作用奠定基础。[ Objective ] This study aimed to analyze and determine antigenic recognition sites, saturation value and relative affinity index of monoclonal antibody against porcine integrin β6 subunit LBD as FMDV receptor. [ Method] Antigenic recognition sites, saturation value and relative affinity index of monoclonal antibody C2D8, B7B6, B8C9, C4C7 and E7C7 were determined by superposition ELISA, indirect ELISA and cyanide sulfonate elution assay. [ Result] Five inter-monoclonal antibodies have identical or different antigenic recognition sites, identical antigenic recognition sites of inter-monoclonal antibodies C4C7 and E7C7, C4C7 and B8C9, E7C7and B8C9 and so on, different ntigenic recognition sites of inter-monoclonal antibodies C4C7 and B7B6, E7C7 and C2D8 and so on. Saturation value of monoclonal antibody C2D8, E7C7, B8C9, CAC7, B7B6 is respectively 1 ： 200, 1 ： 400, 1 ： 800, 1 ： 800, 1 ： 800 and relative affinity index of monoclonal antibody is B8C9 〉 C4C7 〉 B7B6 ≥E7C7 〉 C2D8. [ Conclusion ] This study analyzed and determined antigenic recognition sites, saturation value and relative affinity index of monoclonal antibody, which will lay a foundation to use monoclonal antibody in further understanding of the roles of integrin αvβ6 in FMDV infection.

关 键 词：口蹄疫病毒 整联蛋白 受体 单克隆抗体 抗原表位 

分 类 号：S813.3[农业科学—畜牧学]