抱茎獐牙菜中3种药效成分的HPLC测定  

Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith

作　　者：马玉花[1] 纪兰菊[2] 陈桂琛[2] 卢学峰[2] 

机构地区：[1]青海民族学院化学系,青海西宁810007 [2]中国科学院西北高原生物研究所,青海西宁810001

出　　处：《安徽农业科学》2009年第4期1623-1624,共2页Journal of Anhui Agricultural Sciences

基　　金：国家中西部重点项目(2001BA901A47)

摘　　要：[目的]测定抱茎獐牙菜中不同部位的药效成分。[方法]采用反相高效液相色谱法，测定抱茎獐牙菜中不同部位獐牙菜苦苷、龙胆苦苷及芒果苷的含量。[结果]结果表明，HPLC法测定3种药效成分的最佳条件为流动相甲醇-0．02％磷酸水溶液，梯度洗脱，洗脱条件0～25min，甲醇：20％～50％；流速1ml／min，检测波长为260nm。该方法具有很好的线性关系和回收率。[结论]抱茎獐牙莱根部龙胆苦苷的含量最高，其余2种药效成分在花的部位有较高的含量。[ Objective ] The purpose was to determine three medicinal bioactive components in different parts of Swertiafranchetiana H. Smith. [ Method]The content of swertiamarin, gentiopicroside and mangiferin in Swertia franchetiana H. Smith were determined by reversed-phase high performance liquid chromatography. [ Result] The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H. Smith were as follow ： a Kromasil C18 （ 250 mm × 4.60 mm, 5 μm） ; the mobile phase was composed of CH3 OH and 0.02% phosphoric acid in water; the gradient condition was 0 -25 min, CH3OH 20% -50% ; the flow rate was 1 ml/min; the detection wavelength was 260 nm. The good linear relationships and recoveries were obtained under the optimum conditions. [ Conclusion] The content of gentiopicroside is most high in the root, swertiamarin and mangiferin have higher content in the floral part of Swertiafranchetiana H. Smith.

关 键 词：反相高效液相色谱法 抱茎獐牙菜 獐牙菜苦苷 龙胆苦苷 芒果苷 

分 类 号：S567.239[农业科学—中草药栽培]