毛细管气相色谱法测定牛黄解毒片中冰片含量  

Determination of Borneol Content in Niuhuang Jiedu Tablets by Capillary Gas Chromatography

作　　者：李美月 

机构地区：[1]福建省漳州市药品检验所,福建漳州363000

出　　处：《中国药业》2009年第5期25-26,共2页China Pharmaceuticals

摘　　要：目的建立以气相色谱测定牛黄解毒片中冰片含量的方法。方法色谱柱为HP-5型石英毛细管(30m×0.32mm,0.25μm),样品用乙酸乙酯超声提取。结果冰片检测质量浓度在20.0～500.0μg/mL范围内与峰面积呈良好的线性关系(r=0.9995);平均加样回收率为99.68%,RSD=1.11%(n=9);最低检测限为20pg。结论方法简便、快速、准确,可用于牛黄解毒片的质量控制。Objective To develop a new method of GC assay for the determination of borneol content in Niuhuang Jiedu Tablets. Methods Borneol was analyzed quantitatively on the HP-5 quartz capillary chromatographic column（30 m ×0.32 mm, 0.25 μm）. The sample was extracted by ultrasound in ethyl acetate and quantified by gas chromatography（GC）. Results The good linear relationship was achieved for borneol over the range of 20.0-500.0 μg/mL（r=0.999 5）. The average recovery rate was 99.68%. RSD= 1. 11% （n=9） and the detection limit was 20 pg. Conclusion The method is simple, rapid and accurate, which is suitable for the quality control of borneol in Niuhuang Jiedu Tablets.

关 键 词：毛细管气相色谱 冰片 牛黄解毒片 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]