内生真菌紫杉木霉ZJUF0986菌株及其活性代谢产物防治水稻纹枯病的效果  

Control of Rice Sheath Blight with the Endophytic Fungus ZJUF0986 and Its Bioactive Metabolite

作　　者：王国平[1,2] 鲁书玲[1] 郑必强[1,2] 章初龙[1] 林福呈[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310029 [2]建德市大洋化工有限公司,建德311616

出　　处：《中国生物防治》2009年第1期30-34,共5页Chinese Journal of Biological Control

基　　金：浙江省重大科技攻关项目(2006C12008)

摘　　要：从南方红豆杉分离到的一种新内生真菌紫杉木霉Trichoderma taxi菌株ZJUF0986,与水稻纹枯病菌对峙培养。结果发现,该内生真菌通过菌丝缠绕附着等重寄生方式,导致纹枯病菌菌丝断裂或其内含物降解直至死亡。其产生的活性代谢产物也能强烈抑制纹枯病菌菌丝的生长,显著降低病原菌的菌核萌发率;对病原菌菌丝生长和菌核萌发的有效中浓度EC50分别为1.08和3.59μg/ml。盆栽试验结果表明,菌株ZJUF0986浓度80μg/ml的活性代谢产物对水稻纹枯病的防效达63.82%,与50μg/ml井冈霉素A的防治效果相当。New endophytic fungaus Trichoderma taxi strain ZJUF0986, isolated from Taxus chinensis var. mairei, is of high antifungal activity against various phytopathogenic fungi. ZJUF0986 in dual cuture degraded Rhizoctonia solani hyphae directly when they contacted, through the mode of winding and attachment to pathogen mycelia. Antifungal metabolite produced by ZJUF0986 inhibited pathogen mycelium growth and sclerotia germination with EC50 1.08 μg/ml and 3.59 μg/ml, respectively. Pot trials in greenhouse, showed that antifungal metabolite of ZJUF0986 was effective in control of rice sheath blight. Its control efficacy at 80 μg/ml antifungal metabolite was equal to that of Jinggangmycin-A at 50 μg/ml.

关 键 词：内生真菌 紫杉木霉 水稻纹枯病 拮抗作用 抗菌活性 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治] S476.8[农业科学—植物保护]