一株对香蕉枯萎病菌具有良好拮抗作用的菊欧氏杆菌  

An Endophytic Erwinia chrysanthemi Strain Antagonistic against Banana Fusarium Wilt Disease

作　　者：殷晓敏[1,2] 陈弟[1] 吴红萍[1] 郑服丛[1,3] 

机构地区：[1]海南大学环境与植物保护学院,儋州571737 [2]中国热带农业科学院海口实验站,海口570102 [3]中国热带农业科学院环境与植物保护研究所,儋州571737

出　　处：《中国生物防治》2009年第1期60-65,共6页Chinese Journal of Biological Control

基　　金：中央级公益性科研院所基本科研业务专项资助项目(2007hzs1J011)

摘　　要：在香蕉枯萎病重病园区,从生长正常香蕉假茎内分离获得一株细菌E353菌株。经对峙培养、孢子萌发抑制测定,E353对香蕉枯萎病菌菌丝生长、孢子萌发具有良好抑制效果。盆栽试验表明,E353活菌培养液(750ml/株)浸根处理,对香蕉枯萎病的防效为60.67%。经形态学、生理生化和16S rDNA序列比对,将E353鉴定为菊欧氏杆菌Erwinia chrysanthemi。An endophytic strain E353 was obtained from the pseudostem of healthy banana plant in a field heavily infected with Fusarium oxysporum f. sp. cubense （FOC）. Antagonism of the strain against FOC was tested via dual-culture, inhibition test on conidia germination, and pot trials. Results showed that E353 effectively inhibited mycelium growth and conidia germination. Efficacy of strain E353 to control the wilt disease was 60.67 % in pot trials. Strain E353 was identified as Erwinia chrysanthemi according to its characteristics in morphology, physiology, biochemistry and 16S rDNA sequence.

关 键 词：菊欧氏杆菌 香蕉枯萎病 拮抗作用 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治] S476.8[农业科学—植物保护]