不同品种大豆耐铝性的FTIR分析研究  

FTIR Study of the Endurance to Al of Different Soybean Cultivars

作　　者：张争艳[1] 刘鹏[1] 陈微微[1] 陈传奇[1] 贾佳[1] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004

出　　处：《光谱学与光谱分析》2009年第2期372-377,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30540056);浙江省自然科学基金项目(303461;304185)资助

摘　　要：利用傅里叶变换红外光谱（FTIR）法测定了2个不同耐铝性大豆品种在不同浓度铝处理下根、茎、叶的红外光谱。通过对各谱图的比较分析发现,除928-1200 cm^-1区域的FTIR差异比较明显外,未经处理的2个品种大豆红外光谱基本相似。而铝处理后2个品种大豆根、茎、叶的红外吸收峰形及峰值均与对照有较大的区别,且随铝浓度升高其FTIR区别越为明显。此外,结果表明浙春3号（铝敏感品种）的变化幅度比浙春2号（铝耐性品种）更为明显,这说明铝敏感大豆在铝胁迫下物质代谢受到了较大的影响。因此,采用FTIR法鉴别大豆不同耐铝性能够提供较为准确的结果,具有广泛的应用前景。The spectra of root, stem, leaf of soybean samples with 0, 20, 40, 60 mg · L^-1 Al^3＋ were determined by Fourier transform infrared （F＇FIR） spectrometry with OMNI-sampler. Little difference was found in the spectra of leaf between two soybean cultivars, aluminum-resistant eultivar Zhechun NO. 2 and aluminum-sensitive eultivar Zheehun NO. 3, except the indices af wave number-absorbance from 928 to 1 200 cm^-1 , and similar results were also observed in stem and root samples of the two soybean cultivars with 0 mg · L^-1 Al^3＋. However, results from the comparison of the spectra showed some distinguishable differences in the intensity and the shape of absorption peaks of their FTIR spectra from 721 to 3 366 cm^- 1 of Al-stressed samples and control samples between the two soybean eultivars, and more evident differences of FTIR were exhibited in Al-stressed roots, stems and leaves with higher concentration of Al^3＋. The increased absorbance at 2 929 and 3 350 cm^- 1 was found in root FTIR spectra with 20, 40, 60 mg · L 1 Al^3＋, while roots got maximum absorbance at wave number of 1 375 cm^-1 with 20 mg · L^-1 Al^3＋ , which decreased with higher concentration of Al^3＋ , and the same results were showed at wave numbers of 1 410, 1 423, 1 549 and 1 645 cm^-1. Absorption peak showed maximum at wave numbers of 1 051, 2 850, 2 929 and 3 350 cm^-1 in stem FTIR spectra with 60 mg· L^- 1 Al^3＋. There was little difference between the spectra of Al-stressed leaves and controls at wave numbers from 1 750 to 2 750 cm^-1 , but visible difference in leaf spectra was exhibited at other wave number. Moreover, the results showed that the FFIR spectra of aluminum-sensitive cultivar Zheehun NO. 3 showed much more observable differenees than aluminum-resistant eultivar Zheehun NO. 2 with different concentration of Al, implying that the material metaboly of aluminum-sensitive soybean was evidently affected by Al. Therefore, FTIR spectra could be used broadly for identification of the endurance of different soybea

关 键 词：傅里叶变换红外光谱 大豆 耐铝性 

分 类 号：S565.1[农业科学—作物学]